Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2.
Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2.
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DOI：
10.1038/s41467-021-24613-8
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发表时间：
2021-07-19
影响因子：
16.6
通讯作者：
Snell WJ
中科院分区：
文献类型：
--
作者：
Zhang J;Pinello JF;Fernández I;Baquero E;Fedry J;Rey FA;Snell WJ
Recognition and fusion between gametes during fertilization is an ancient process. Protein HAP2, recognized as the primordial eukaryotic gamete fusogen, is a structural homolog of viral class II fusion proteins. The mechanisms that regulate HAP2 function, and whether virus-fusion-like conformational changes are involved, however, have not been investigated. We report here that fusion between plus and minus gametes of the green alga Chlamydomonas indeed requires an obligate conformational rearrangement of HAP2 on minus gametes from a labile, prefusion form into the stable homotrimers observed in structural studies. Activation of HAP2 to undergo its fusogenic conformational change occurs only upon species-specific adhesion between the two gamete membranes. Following a molecular mechanism akin to fusion of enveloped viruses, the membrane insertion capacity of the fusion loop is required to couple formation of trimers to gamete fusion. Thus, species-specific membrane attachment is the gateway to fusion-driving HAP2 rearrangement into stable trimers. HAP2 is essential for gamete fusion during fertilization and is conserved among eukaryotes. Here the authors show that species-specific adhesion between Chlamydomonas plus and minus gametes initiates HAP2 to undergo a fusogenic conformational change into homotrimers via a molecular mechanism akin to that of enveloped viruses.
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影响因子：
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作者：
Hussein HE;Bastos RG;Schneider DA;Johnson WC;Adham FK;Davis WC;Laughery JM;Herndon DR;Alzan HF;Ueti MW;Suarez CE
通讯作者：
Suarez CE
影响因子：
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Springer, Timothy A.
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作者：
Baquero, Eduard;Fedry, Juliette;Rey, Felix A.
通讯作者：
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