The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino)
The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino)
复制标题
不结球白菜遗传连锁图谱的构建
DOI：
10.1016/s1673-8527(08)60140-x
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发表时间：
2009-08-01
影响因子：
5.9
通讯作者：
Hou, Xilin
中科院分区：
文献类型：
--
作者：
Cheng, Yan;Geng, Jianfeng;Hou, Xilin
不结球白菜（Brassica campestris ssp.）chinensis Makino）是我国东部地区最重要的蔬菜之一。利用127个双单倍体（DH）品系构建了遗传连锁图谱，DH群体来自于商品杂交种“旱笑”（SW-13 × L-118）。在614个多态性标记中，有43.49%的标记没有被分配到任何连锁群（LGs）。卡方检验表明，有42.67%的标记偏离了孟德尔分离比例，偏离的方向主要是偏向父本L-118。在依次去除与tipper标记间隔距离小于1cM的标记后，连锁图的整体质量得到提高。将268个分子标记定位到10个LGS上，并将其锚定在B的相应染色体上。根据常见的简单重复序列（SSR）标记构建了芜菁参考图谱。该图谱覆盖了973.38 cM的基因组，标记间的平均间隔距离为3.63 cM。每个LG上的标记数为18（R 08）~ 64（R 07），单个LG内的平均间隔距离为1.70 cM（R 07）~ 6.71 cM（R 06）。其中，序列相关扩增多态性（SRAP）标记169个，SSR标记50个，随机扩增多态性DNA（RAPD）标记49个。随着LG的进一步饱和，目前的地图提供了一个重要的农艺性状的基因座分析的遗传工具。
Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is one of the most important vegetables in eastern China. A genetic linkage map was constructed using 127 doubled haploid (DH) lines, and the DH population was derived from a commercial hybrid "Hanxiao" (lines SW-13 x L-118). Out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups (LGs). Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios, and the direction of distorted segregation was mainly toward the paternal parent L-118. After sequentially removing the markers that had an interval distance smaller than 1 cM from the tipper marker, the overall quality of the linkage map was increased. Two hundred and sixty-eight molecular markers were mapped into 10 LGs, which were anchored to the corresponding chromosome of the B. rapa reference map based on common simple sequence repeat (SSR) markers. The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM. The number of markers on each LG ranged from 18 (R08) to 64 (R07), with an average interval distance within a single LG from 1.70 cM (R07) to 6.71 cM (R06). Among these mapped markers, 169 were sequence-related amplified polymorphism (SRAP) molecular markers, 50 were SSR markers and 49 were random amplification polymorphic DNA (RAPD) markers. With further saturation to the LG, the current map offers a genetic tool for loci analysis for important agronomic traits.