Identification and characterization of Huntington related pathology: An in vivo DKI imaging study
Identification and characterization of Huntington related pathology: An in vivo DKI imaging study
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DOI：
10.1016/j.neuroimage.2012.06.032
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发表时间：
2012-11-01
期刊：
影响因子：
5.7
通讯作者：
Van der Linden, Annemie
中科院分区：
文献类型：
--
作者：
Blockx, Ines;Verhoye, Marleen;Van der Linden, Annemie
亨廷顿病（HD）研究的一个重要焦点是HD神经病理学的非亨廷顿依赖性生物标志物的鉴定。目前迫切需要可重复的、敏感的和特异性的结局指标，这些指标可用于跟踪疾病的发作和进展。神经影像学研究，特别是基于扩散的MRI方法，是表征疾病和衰老对组织微观结构影响的有力探针。我们报告新的扩散峰度成像（DKI）的结果在老年转基因HD大鼠。我们证明了改变扩散指标（前）额叶大脑皮层，外囊和纹状体。纹状体中扩散复杂性增加和限制的存在通过该区域中纤维分散增加来证实。相同标本的免疫染色显示（前）额叶皮质中的小胶质细胞数量减少，纹状体中的少突胶质细胞数量增加。我们的结论是，DKI允许在HD大鼠模型中改变组织完整性的灵敏和特异性表征，表明灰色和白色物质病理学的诊断成像的有前途的潜力。(C)2012 Elsevier Inc. All rights reserved.
An important focus of Huntington Disease (HD) research is the identification of symptom-independent biomarkers of HD neuropathology. There is an urgent need for reproducible, sensitive and specific outcome measures, which can be used to track disease onset as well as progression. Neuroimaging studies, in particular diffusion-based MRI methods, are powerful probes for characterizing the effects of disease and aging on tissue microstructure. We report novel diffusional kurtosis imaging (DKI) findings in aged transgenic HD rats. We demonstrate altered diffusion metrics in the (pre)frontal cerebral cortex, external capsule and striatum. Presence of increased diffusion complexity and restriction in the striatum is confirmed by an increased fiber dispersion in this region. Immunostaining of the same specimens reveals decreased number of microglia in the (pre)frontal cortex, and increased numbers of oligodendrocytes in the striatum. We conclude that DKI allows sensitive and specific characterization of altered tissue integrity in this HD rat model, indicating a promising potential for diagnostic imaging of gray and white matter pathology. (C) 2012 Elsevier Inc. All rights reserved.