EFFECT OF INHALED ENDOTOXIN ON BRONCHIAL REACTIVITY IN ASTHMATIC AND NORMAL SUBJECTS
EFFECT OF INHALED ENDOTOXIN ON BRONCHIAL REACTIVITY IN ASTHMATIC AND NORMAL SUBJECTS
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DOI：
10.1152/jappl.1989.66.3.1059
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发表时间：
1989-03-01
影响因子：
3.3
通讯作者：
SERGYSELS, R
中科院分区：
文献类型：
--
作者：
MICHEL, O;DUCHATEAU, J;SERGYSELS, R
内毒素是从存在于环境中以及口腔和鼻腔中的革兰氏阴性菌的膜释放的。它们是促炎物质，可能参与哮喘患者的支气管阻塞和高反应性。通过使用来自大肠杆菌026：B6的吸入性脂多糖（LPS）（22.2 μ g总剂量）的支气管激发试验，随后进行组胺非特异性激发试验，并与安慰剂程序（其中稀释剂代替LPS溶液）进行比较，来检验该假设。在此过程中，我们发现LPS在哮喘患者（n = 8）中诱导轻微但显著（P < 0.01）的支气管阻塞（以1秒用力呼气量测量），但在正常受试者（n = 6）中则无此现象。哮喘患者吸入LPS后5 h，使支气管比传导降低50%所需的组胺量明显增加（P < 0.05），而正常人则无明显变化。在外源性（n = 6）和内源性（n = 2）哮喘中观察到LPS对支气管阻塞和高反应性的影响。
Endotoxins are released from the membrane of Gram-negative bacteria present in the environment and in oral and nasal cavities. They are proinflammatory substance that could participate in bronchial obstruction and hyperreactivity in asthmatic patients. This hypothesis was tested by using bronchial challenge tests with inhaled lipopolysaccharides (LPS) from Escherichia coli 026:B6 (22.2-.mu.g total dose) followed by a histamine nonspecific challenge test and compared with a placebo procedure, in which the diluent was substituted for the LPS solution. In doing so we showed that LPS induces a slight but significant (P < 0.01) bronchial obstruction (measured as forced expiratory volume in 1 s) in asthmatics (n = 8) but not in normal subjects (n = 6). The histamine hyperresponsiveness, expressed as the dosage of histamine necessary to decrease the bronchial specific conductance by 50%, was increased 5 h after LPS inhalation in asthmatics (P < 0.05) but not in normal subjects. This effect of LPS on bronchial obstruction and hyperresponsiveness was observed in extrinsic (n = 6) as well as in intrinsic (n = 2) asthma.