Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection
Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection
复制标题
DOI：
10.1002/jmv.10206
复制
发表时间：
2002-11-01
影响因子：
12.7
通讯作者：
Margolis, HS
中科院分区：
文献类型：
--
作者：
Nainan, OV;Khristova, ML;Margolis, HS
已在许多慢性B型肝炎病毒（HBV）感染人群中发现了B型肝炎表面抗原（HBsAg）主要抗体结合位点氨基酸组成的变异体。比较扩增或克隆PCR产物的直接测序、序列特异性PCR产物的固相检测（SP-PCR）和有限稀释克隆PCR（LDC-PCR），以确定它们检测不同浓度HBsAg变体的灵敏度。LDC-PCR具有最高的灵敏度，可以检测到总病毒群浓度为0.1%的HBsAg变异体。在51%的慢性HBV感染婴儿中检测到HBsAg变异体，这些变异体只有通过最敏感的方法才能检测到。(C)2002 Wiley-Liss，Inc.
Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods. (C) 2002 Wiley-Liss, Inc.