Polyphasic Taxonomic Study of the Emended Genus Comamonas: Relationship to Aquaspirillum aquaticum, E. Falsen Group 10, and Other Clinical Isolates
Polyphasic Taxonomic Study of the Emended Genus Comamonas: Relationship to Aquaspirillum aquaticum, E. Falsen Group 10, and Other Clinical Isolates
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改良丛毛单胞菌属的多相分类学研究：与水螺菌、E. Falsen Group 10 和其他临床分离株的关系
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
J. Ley
中科院分区：
文献类型：
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作者：
A. Willems;B. Pot;E. Falsen;P. Vandamme;M. Gillis;K. Kersters;J. Ley
我们采用DNA-rRNA杂交、DNA碱基组成、全细胞蛋白质聚丙烯酰胺凝胶电泳、DNA-DNA杂交、表型特征数值分析和免疫分型等方法对丛毛单胞菌属进行了分类研究。本属与水生螺菌（Aquaspirillum aquaticum）和一组临床分离株（E. Falsen组（EF 10）。DNA和rRNA杂交结果表明，丛毛单胞菌属至少由以下5个基因型群组成：（i）食酸丛毛单胞菌（Comamonasacidovorans），（ii）睾丸酮丛毛单胞菌（Comamonastestosteroni），（iii）土丛毛单胞菌（Comamonasterrigena），（iv）嗜酸丛毛单胞菌（A.水生假单胞菌和许多EF 10菌株，和（v）其他EF 10菌株，几个未命名的临床分离株，和一些误称的产碱假单胞菌和假产碱假单胞菌亚种菌株。假阿拉利基因免疫分型和蛋白质凝胶电泳结果证实了这五组的存在。数值分析的形态，auxanographic，和生化数据相同的生物体揭示了存在三个大现象。其中两个phena（C. acidovorans和C. testosteroni）对应于两个基因型组。第三个表型包括属于其他三个基因型群的菌株，包括大多数EF 10菌株和C. terrigena和A.水生植物属于第三种现象的菌株均整合到C. terrigena，我们建议停止使用Aquaspirillum aquaticum这个名称。对丛毛单胞菌属和丛毛单胞菌C. terrigena介绍。
We used DNA-rRNA hybridization, DNA base composition, polyacrylamide gel electrophoresis of whole-cell proteins, DNA-DNA hybridization, numerical analysis of phenotypic features, and immunotyping to study the taxonomy of the genus Comamonas. The relationships of this genus to Aquaspirillum aquaticum and a group of clinical isolates (E. Falsen group 10 [EF 10]) were studied. Our DNA and rRNA hybridization results indicate that the genus Comamonas consists of at least the following five genotypic groups: (i) Comamonas acidovorans, (ii) Comamonas testosteroni, (iii) Comamonas terrigena, (iv) A. aquaticum and a number of EF 10 strains, and (v) other EF 10 strains, several unnamed clinical isolates, and some misnamed strains of Pseudomonas alcaligenes and Pseudomonas pseudoalcaligenes subsp. pseudoalaligenes. The existence of these five groups was confirmed by the results of immunotyping and protein gel electrophoresis. A numerical analysis of morphological, auxanographic, and biochemical data for the same organisms revealed the existence of three large phena. Two of these phena (C. acidovorans and C. testosteroni) correspond to two of the genotypic groups. The third phenon contains strains belonging to the other three genotypic groups, including most EF 10 strains and the type strains of C. terrigena and A. aquaticum. The strains belonging to the third phenon were all incorporated into C. terrigena, and we propose that the use of the name Aquaspirillum aquaticum should be discontinued. Emended descriptions of the genus Comamonas and C. terrigena are presented.