Study on Genetic Diversity of T128 del in Agouti Gene Intron 1 in Chinese Main Indigenous Goat Breeds
Study on Genetic Diversity of T128 del in Agouti Gene Intron 1 in Chinese Main Indigenous Goat Breeds
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发表时间：
2009
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通讯作者：
Wang Jian-tao
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作者：
Wang Jian-tao
Agouti基因在家畜色素合成中发挥着重要作用，通过测序发现了一个SNP-T128 del。通过PCR-RFLP分析了10个中国地方山羊品种T128 del的遗传多样性，并分析了基因型与毛色的关系。检测到A（non-del）和B（del）两个等位基因，AA、AB和BB 3种基因型。AA基因型在棕色毛色方面表现优异山羊品种中(77.41%);AB基因型主要存在于白毛色山羊品种(76.14%)中,黑毛色山羊品种中BB基因型居多(87.92%),推测T128 del可能对山羊色素合成有影响。10个山羊品种中T128 del的预期杂合度、基因流和平均基因分化系数为分别为0.471 8、0.319 0和0.439 3。结果表明，本文调查的大多数山羊品种在T128 del遗传多样性较差。
Agouti gene plays an important role in the pigment synthesis of domestic animals.A SNP-T128 del was found by sequencing.The genetic diversity of T128 del in ten Chinese indigenous goat breeds was analyzed,as well as the relationship between the genotypes and the coat color by PCR-RFLP.Two alleles of A(non-del) and B(del),three genotypes of AA,AB and BB were detected.The genotype AA was superior in brown coat color goat breeds(77.41%);genotype AB existed mainly in white coat color goat breeds(76.14%) and the goat breeds with black coat color had BB genotype mostly(87.92%),so it was deduced that the T128 del might has the effect on the pigment synthesis of goat.The expected heterozygosity,the gene flow and the mean coefficient of gene differentiation of T128 del among ten goat breeds were 0.471 8,0.319 0 and 0.439 3,respectively.The results indicated that most goat breeds investigated here were poor in genetic diversity at T128 del.