Surveying CpG methylation at 5′-CCGG in the genomes of rice cultivars
Surveying CpG methylation at 5′-CCGG in the genomes of rice cultivars
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DOI：
10.1023/a:1006457321781
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发表时间：
2001-01-01
影响因子：
5.1
通讯作者：
Ashikawa, I
中科院分区：
文献类型：
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作者：
Ashikawa, I
我们利用甲基化敏感的AFLP技术和Southern杂交技术研究了水稻基因组中CCGG序列的甲基化状态。位于单拷贝或低拷贝序列区域的CpG可根据其甲基化状态分为两类：第一类CpG位点的甲基化状态在遗传多样的水稻品种中是保守的，而第二类CpG位点的品种特异性差异甲基化在品种中经常被检测到。一对品种间甲基化多态性的出现频率与品种间的遗传距离无关。通过作图，5个第2类CpG位点位于不同的染色体上，并且在基因组中不聚在一起。分离分析的品种特异性甲基化与其目标位点表明，差异甲基化是稳定遗传的孟德尔式超过6代，虽然在2类CpG位点的甲基化状态的改变，观察到的频率较低。
We investigated the CpG methylation status of the sequence CCGG in the rice genome by using methylation-sensitive AFLP and subsequent Southern analyses with the isolated AFLP fragments as probes. CpGs located in single- or low-copy-sequence regions could be grouped into two classes on the basis of their methylation status: methylation status at the class 1 CpG sites was conserved among genetically diverse rice cultivars, whereas cultivar-specific differential methylation was frequently detected among the cultivars at the class 2 CpG sites. The frequency of occurrence of methylation polymorphism between a pair of cultivars was not related to the genetic distance between the two. Through mapping, five class 2 CpG sites were localized on different chromosomes and were not clustered together in the genome. Segregation analysis of the cultivar-specific methylations with their target sites indicated that the differential methylation was stably inherited in a Mendelian fashion over 6 generations, although alterations in the methylation status at the class 2 CpG sites were observed with a low frequency.