Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi
Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi
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DOI：
10.1016/s0168-1656(01)00285-1
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发表时间：
2001-07-12
影响因子：
4.1
通讯作者：
Tan, RX
中科院分区：
文献类型：
--
作者：
Liu, CH;Zou, WX;Tan, RX
以生产抗疟疾药物青蒿素而闻名的黄花蒿很少受到任何植物致病真菌的攻击，这可能与内生菌的存在部分相关。本研究旨在揭示黄花蒿内的内生菌是否产生抗真菌物质。对39株内生菌进行了分离发酵，并对发酵液进行了体外抑菌活性评价，测定了发酵液对威胁作物的几种真菌的抑菌活性，包括：小麦芽胞菌、小麦根丝胞菌、小麦Helminthosporium sativum、谷物镰刀菌、Gerlachia nicalis和辣椒疫霉。这些植物病原体仍分别引起小麦全蚀病、尖眼斑病、普通腐病、痂病、雪霉病和辣椒疫霉病。在所研究的39种内生菌中，21种可以在体外产生对所有或少数被测植物病原体具有抑制作用的物质，而其余的则不产生任何活性。此外，用乙酸乙酯和正丁醇提取内生菌IV403的肉汤活性最高，并对两种提取物的抑菌活性进行了比较，结果表明乙酸乙酯是内生菌IV403的主要活性代谢产物。(C) 2001 Elsevier Science B.V.版权所有
Artemisia annua, well recognized for its production of antimalarial drug artemisinin, is seldom attacked by any of phytopathogenic fungi, which could be partially associated with the presence of endophytes. Present investigation is aiming at disclosing whether the endophytes inside A. annua produce antifungal substances. A total of 39 endophytes were isolated and fermented, and the ferment broth was evaluated in vitro for the antifungal activity against crop-threatening fungi Gaeumannomyces graminis var. tritici, Rhizoctonia cerealis, Helminthosporium sativum, Fusarium graminearum, Gerlachia nicalis and Phytophthora capsici. These plant pathogens are still causing wheat take-all, sharp eyespot, common rot, scab, snow mould, and pepper phytophthora blight, respectively. Out of 39 endophytes investigated, 21 can produce in vitro substances that are inhibitory to all or a few of the tested phytopathogens whereas the rest yielded nothing active. Moreover, the most active broth of endophyte IV403 was extracted with EtOAc and n-butanol, and comparisons of the antifungal activity of the extracts indicated that the major active metabolites were EtOAc-extractable. (C) 2001 Elsevier Science B.V. All rights reserved.