Hypoxia enhances the expression of follistatin-like 3 in term human trophoblasts
Hypoxia enhances the expression of follistatin-like 3 in term human trophoblasts
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DOI：
10.1016/j.placenta.2007.09.001
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发表时间：
2008-01-01
期刊：
影响因子：
3.8
通讯作者：
Sadovsky, Y.
中科院分区：
文献类型：
--
作者：
Biron-Shental, T.;Schaiff, W. T.;Sadovsky, Y.
缺氧损伤阻碍胎盘分化并改变滋养细胞基因表达。我们验证了卵泡抑素家族蛋白成员卵泡抑素样3 （FSTL3）的表达在原代人滋养细胞（PHT）中受缺氧调节的假设。利用人足月胎盘绒毛的免疫荧光检测FSTL3蛋白在胎盘绒毛中的表达，主要是在滋养细胞中。我们在培养的PHT细胞中证实了这一发现。在培养的PHT细胞中，FSTL3转录物的基础表达在培养期间是稳定的。重要的是，与FiO(2) = 20%或FiO(2) = 8%的培养液相比，FiO(2)中培养的PHT细胞
Hypoxic injury hinders placental differentiation and alters trophoblast gene expression. We tested the hypothesis that the expression of follistatin-like 3 (FSTL3), a member of the follistatin family of proteins, is modulated by hypoxia in primary human trophoblast (PHT). Using immunofluorescence of human term placental villi we detected the expression of FSTL3 protein in placental villi, primarily in trophoblasts. We verified this finding in cultured term PHT cells. Basal expression of FSTL3 transcript in cultured PHT cells, determined using quantitative PCR, was stable over the culture period. Importantly, when compared to culture in FiO(2) = 20% or FiO(2) = 8%, PHT cells cultured in FiO(2)