Characterization of cell matrix associated collagens synthesized by aortic endothelial cells in culture.
Characterization of cell matrix associated collagens synthesized by aortic endothelial cells in culture.
复制标题
培养物中主动脉内皮细胞合成的细胞基质相关胶原蛋白的表征。
DOI：
10.1021/bi00505a032
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Bornstein,P
中科院分区：
文献类型：
--
作者：
Sage,H;Pritzl,P;Bornstein,P
摘要：从牛主动脉内皮细胞及其相关的细胞外基质中分离并鉴定了几种类型的胶原蛋白。两种胶原蛋白与al (III), al (V)和a2 (V)胶原蛋白链在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上共迁移，通过盐沉淀从细胞层蛋白的胃蛋白酶消化中分离出来。其中两条链通过分子筛和离子交换色谱进一步纯化，并通过肥大细胞蛋白酶和氰生成的一维肽图鉴定为al （III）和al (V)
Helene Sage, Pam Pritzl, and PaulBornstein* abstract: Several collagen types have been isolated and characterized from bovine aortic endothelial cells and their associated extracellular matrix. Two collagens, which co-migrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with the al (III), al (V), and a2 (V) collagen chains, were isolated by salt precipitationfrom pepsin digests of cell layer proteins. Two of these chains were further purified by molecular-sieve and ion-exchange chromatography and were identified as al (III) and al (V) by one-dimensional peptide maps generated with mast cell protease and cyanogen