A nanozyme-linked immunosorbent assay based on metal-organic frameworks (MOFs) for sensitive detection of aflatoxin B1.
A nanozyme-linked immunosorbent assay based on metal-organic frameworks (MOFs) for sensitive detection of aflatoxin B1.
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DOI：
10.1016/j.foodchem.2020.128039
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发表时间：
2020-09
期刊：
影响因子：
8.8
通讯作者：
Zhou Xu;Ling-li Long;Yan-qiu Chen;Maolong Chen;Yunhui Cheng
中科院分区：
文献类型：
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作者：
Zhou Xu;Ling-li Long;Yan-qiu Chen;Maolong Chen;Yunhui Cheng
为了避免常规酶联免疫吸附试验（ELISA）中假阳性和假阴性的发生，建立了一种新的高通量、高灵敏度检测黄曲霉毒素B1的间接竞争性MOF联免疫吸附试验（MOFLISA）。该方法用功能性MOFs代替天然酶来催化显色体系。因此，MOFLISA方法的检测限（LOD）为0.009 ng·mL− 1，线性工作范围为0.01 - 20 ng·mL−1。与常规ELISA法相比，MOFLISA法检测黄曲霉毒素B1的检出限提高了20倍。加标回收率为86.41 ~ 99.74%，相对标准偏差（RSD）为2.38- 9.04%。结果表明，该检测方法的回收率和准确性均优于常规ELISA，降低了假阳性和假阴性的风险。
In order to avoid the occurrence of false positives and false negatives caused by conventional enzyme-linked immunosorbent assay (ELISA), we established a novel indirect competitive MOF-linked immunosorbent assay (MOFLISA) method for the high throughput and high sensitive detection of aflatoxin B1. This method replaces the natural enzyme with functional MOFs to catalyze a chromogenic system. As a result, the limit of detection (LOD) of the MOFLISA method was 0.009 ng·mL−1with a linear working range from 0.01 to 20 ng·mL−1. The developed MOFLISA method for AFB1has a 20-fold improved LOD value compared with the conventional ELISA. The recoveries and relative standard deviations (RSD) ranged from 86.41 to 99.74% and 2.38–9.04%, respectively. The results demonstrate that the recovery rate and accuracy of this detection method is better than that of conventional ELISA, reducing risks of false positive and false negative results.