Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion
Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion
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DOI：
10.1089/107632700300003297
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发表时间：
2001-02-01
影响因子：
--
通讯作者：
Jacobs, JJ
中科院分区：
文献类型：
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作者：
Hallab, NJ;Bundy, KJ;Jacobs, JJ
细胞定向粘附是植入和组织工程技术的重要目标。在这项研究中，表面能和表面粗糙度进行了调查，以确定这些属性显示更全面的影响生物材料细胞粘附和定植。射流冲击用于量化细胞粘附强度。使用细胞增殖和细胞外基质分泌来表征3 T3 MC成纤维细胞在以下材料上的定殖：HS 25（钴基植入物合金，ASTM F75）、316 L不锈钢、Ti-6Al-4V（钛植入物合金）、商业纯钽（Ta）、聚四氟乙烯（PTFE）、硅橡胶（SR）和高密度聚乙烯（HDPE）。金属表现出比测试的聚合物材料高近五倍的粘附强度。一般来说，表面能与细胞粘附强度成正比。只有聚合物材料表现出显著增加的粘附强度与增加的表面粗糙度。细胞在金属上的粘附力与表面能呈线性相关。与金属相比，在聚合物材料上检测到的细胞增殖不到一半。然而，所测试的聚合物显示出比金属材料高两倍以上的分泌的细胞外基质（ECM）蛋白质的量（以每单位计）。因此，表面能可能是细胞粘附和增殖的更重要的决定因素，并且可能比表面粗糙度更有助于引导细胞粘附和细胞定植到工程化组织支架上。
Directed cell adhesion remains an important goal of implant and tissue engineering technology. In this study, surface energy and surface roughness were investigated to ascertain which of these properties show more overall influence on biomaterial-cell adhesion and colonization. Jet impingement was used to quantify cellular adhesion strength. Cellular proliferation and extracellular matrix secretion were used to characterize colonization of 3T3MC fibroblasts on: HS25 (a cobalt based implant alloy, ASTM F75), 316L stainless steel, Ti-6Al-4V (a titanium implant alloy), commercially pure tantalum (Ta), polytetrafluoroethylene (PTFE), silicone rubber (SR), and high-density polyethylene (HDPE). The metals exhibited a nearly five-fold greater adhesion strength than the polymeric materials tested. Generally, surface energy was proportional to cellular adhesion strength. Only polymeric materials demonstrated significant increased adhesion strength associated with increased surface roughness. Cellular adhesion on metals demonstrated a linear correlation with surface energy. Less than half as much cellular proliferation was detected on polymeric materials compared to the metals. However the polymers tested demonstrated greater than twice the amount of secreted extracellular matrix (ECM) proteins on a per tell basis than the metallic materials. Thus, surface energy may be a more important determinant of cell adhesion and proliferation, and may be more useful than surface roughness for directing cell adhesion and cell colonization onto engineered tissue scaffoldings.