CHARACTERISTICS OF AEDES-ALBOPICTUS CELLS PERSISTENTLY INFECTED WITH DENGUE VIRUSES
CHARACTERISTICS OF AEDES-ALBOPICTUS CELLS PERSISTENTLY INFECTED WITH DENGUE VIRUSES
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DOI：
10.1038/280690a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
IGARASHI, A
中科院分区：
文献类型：
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作者：
IGARASHI, A
登革病毒有四种血清型，属于披膜病毒科黄病毒属，由埃及伊蚊、白纹伊蚊等蚊子传播。人感染登革病毒表现为严重程度不同的疾病，从轻度的未分化热到严重的出血热，在东南亚广泛传播1 -3。当培养的蚊子细胞被披膜病毒科（甲病毒或黄病毒）感染时，可以建立持续感染的培养物4 -7。对持续感染甲病毒的蚊子细胞的分析显示了一些特征，如产生小噬斑和温度敏感（ts）病毒7 -9，以及对同源病毒而非异源病毒的重复感染的抗性6，10。相比之下，很少有人知道蚊子细胞持续感染黄病毒4，11，12。在这里，在实验与A。白纹伊蚊（辛格）细胞持续感染登革热病毒，我表明，这种文化产生的ts病毒，并成为抵抗所有类型的登革热病毒。
DENGUE VIRUSES, which have four serotypes, belong to the genus flavivirus of Togaviridae and are transmitted by mosquitoes such asAedes aegyptiorAedes albopictus. Human infection with dengue viruses is manifested by disease varying in severity from mild undiflferentiated fever to grave haemorrhagic fever, which is widespread in Southeast Asia1–3. Persistently infected cultures can be established when cultured mosquito cells are infected with Togaviridae, either alphaviruses or flavi viruses4–7. Analysis of mosquito cells persistently infected with alphaviruses has shown characteristics such as the generation of small-plaque and temperature-sensitive (ts) viruses7–9, and resistance to superinfections with the homologous but not the heterologous viruses6,10. In contrast, little is known about mosquito cells persistently infected with flavi viruses4,11,12. Here, in experiments withA. albopictus(Singh) cells persistently infected with dengue viruses, I show that such cultures produce ts viruses and become resistant to all the types of dengue viruses.