Engineering Thiolated Surfaces with Polyelectrolyte Multilayers
Engineering Thiolated Surfaces with Polyelectrolyte Multilayers
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DOI：
10.1021/acsami.8b15514
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发表时间：
2019-01-23
影响因子：
9.5
通讯作者：
Schlenoff, Joseph B.
中科院分区：
文献类型：
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作者：
Delgado, Jose D.;Surmaitis, Richard L.;Schlenoff, Joseph B.
带有牢固连接的硫醇基团的界面可用于许多需要多功能且易于化学反应的-SH功能的应用。在这项工作中，采用逐层组装的方式在基底上制备了坚固的超薄膜。这些光滑薄膜的表面被含有苄硫醇单元的共聚物封端。通过三个应用说明了这种涂层的实用性。首先，使用硫醇-烯“点击”化学在表面上引入精氨酸-甘氨酸-天冬氨酸（RGD）粘附肽序列，否则该表面会阻碍成纤维细胞的良好粘附。这种处理促进了细胞粘附和扩散。采用类似的迈克尔加成化学将聚乙二醇附着到表面，这减少了血清白蛋白（粘附）造成的污垢。最后，通过在硫醇化表面上沉积一层金纳米颗粒或通过蒸发一层坚韧的金膜而不使用经典的铬“底漆”层，利用金对SH的亲和力。
Interfaces bearing firmly attached thiol groups are useful for many applications requiring the versatile and facile chemistry of the -SH functionality. In this work, rugged ultrathin films were prepared on substrates using layer-by-layer assembly. The surface of these smooth films was capped with a co-polymer containing benzyl mercaptan units. The utility of this coating was illustrated by three applications. First, thiol-ene "click" chemistry was used to introduce the Arg-Gly-Asp (RGD) adhesive peptide sequence on a surface that otherwise resisted good adhesion of fibroblasts. This treatment promoted cell adhesion and spreading. Similar Michael addition chemistry was employed to attach poly(ethylene glycol) to the surface, which reduced fouling by (adhesion of) serum albumin. Finally, the affinity of gold for SH was exploited by depositing a layer of gold nanoparticles on the thiolated surface or by evaporating a tenacious film of gold without using the classical chromium "primer" layer.