Effect of a New Titanium Coating Material (CaTiO3-aC) Prepared by Thermal Decomposition Method on Osteoblastic Cell Response
Effect of a New Titanium Coating Material (CaTiO3-aC) Prepared by Thermal Decomposition Method on Osteoblastic Cell Response
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DOI：
10.1177/0885328209340334
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Suzuki, Kazuomi
中科院分区：
文献类型：
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作者：
Inoue, Miho;Rodriguez, Andrea P.;Suzuki, Kazuomi
钛和羟基磷灰石(HA)被广泛用作牙科和医疗领域的生物材料。羟基磷灰石涂层钛种植体具有良好的生物相容性和力学性能。然而，由于羟基磷灰石与钛之间缺乏化学作用，在钛表面形成的羟基磷灰石薄膜的附着力较弱。解决这一问题的一种方法是在钛衬底上形成一层中间膜，使其与钛衬底和羟基磷灰石具有良好的粘附性。采用改进的热分解法制备了一种新型生物材料钛酸钙-无定形碳(CaTiO3-Ac)涂层。本研究的目的是评价CaTiO_3-Ac和HA涂层(阳性对照)和钛(阴性对照)对成骨细胞(MT3T3-E1)细胞反应的影响。与HA涂层相比，CaTiO_3-Ac涂层具有更强的细胞增殖能力。CaTiO_3-Ac涂层的ALP活性、Col I和ALP m RNA的最大表达较高，且在较短的时间内达到最大值。这些结果表明，与HA相比，CaTiO_3-Ac具有更好的细胞附着、细胞增殖和成骨分化作用。综上所述，我们认为CaTiO_3-Ac可以被认为是一种重要的涂层材料。
Titanium and hydroxyapatite (HA) are widely used as biomaterials for dental and medical applications. HA-coated titanium implants have excellent biocompatibility and mechanical properties. However, the adherence of HA film formed on titanium substrate is weak because of the lack of chemical interaction between HA and titanium. A solution to this problem is to form an intermediate film on titanium substrate, which provide excellent adherence to both titanium substrate and HA. We developed a novel biomaterial called calcium titanate-amorphous carbon (CaTiO3-aC) coating prepared by modified thermal decomposition-method. The purpose of this study was to evaluate the effect of CaTiO3-aC and HA coating (positive control), and Ti (negative control) on osteoblastic (MT3T3-E1) cell responses. An increased cellular proliferation was observed in CaTiO3-aC coating compared to HA coating. The maximum expressions of ALP activity, Col I and ALP mRNA were higher and achieved in shorter period of time in CaTiO3-aC coating compared to others. These results demonstrated that CaTiO3-aC promoted better cell attachment, cellular proliferation, and osteoblastic differentiation compared with HA. In conclusion, we suggested that CaTiO3-aC could be considered as an important candidate as a coating material.