Efficacy of Bacteriophage Treatment in Murine Burn Wound Infection Induced by Klebsiella pneumoniae
Efficacy of Bacteriophage Treatment in Murine Burn Wound Infection Induced by Klebsiella pneumoniae
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DOI：
10.4014/jmb.0808.493
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发表时间：
2009-06-01
影响因子：
2.8
通讯作者：
Chhibber, Sanjay
中科院分区：
文献类型：
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作者：
Kumari, Seema;Harjai, Kusum;Chhibber, Sanjay
在本研究中，对纯化和特性良好的噬菌体在感染肺炎克雷伯菌B5055的热损伤小鼠中的治疗潜力进行了评估。对5种克雷伯氏菌噬菌体(Kpn5、Kpn12、Kpn13、Kpn17和Kpn22)的疗效进行了评价，观察了5种克雷伯菌噬菌体对动物不同器官的存活率、不同器官中细菌数量的减少以及皮肤细胞的再生。对所有噬菌体进行的毒性研究表明，它们是无毒的，因为在接受噬菌体处理的小鼠中没有观察到发病和死亡的迹象。研究结果表明，单次腹腔注射噬菌体对小鼠有明显的抑制作用。在MOI为1.0时，可显著降低死亡率，该剂量被发现足以完全治愈烧伤创面模型中的肺炎克雷伯菌感染。感染后72h，各脏器细菌数量下降幅度最大。经噬菌体处理的小鼠皮肤组织病理学检查显示，烧伤感染已完全恢复。在所有噬菌体中，Kpn5噬菌体被发现是高效的，并且与所有噬菌体的鸡尾酒一样有效。从这些结果可以得出结论，噬菌体疗法有可能作为肺炎克雷伯菌引起的烧伤创面感染的独立治疗方法，特别是在遇到多重耐药微生物的情况下。
In the present study, the therapeutic potential of purified and well-characterized bacteriophages was evaluated in thermally injured mice infected with Klebsiella pneumoniae B5055. The efficacy of five Klebsiella phages (Kpn5, Kpn12, Kpn13, Kpn17, and Kpn22) was evaluated on the basis of survival rate, decrease in bacterial counts in different organs of phage-treated animals, and regeneration of skin cells as observed by histopathological examination of phage-treated skin. Toxicity studies performed with all the phages showed them to be non-toxic, as no signs of morbidity and mortality were observed in phage-treated mice. The results of the study indicate that a single dose of phages, intraperitoneally (i.p.) at an MOI of 1.0, resulted in significant decrease in mortality, and this dose was found to be sufficient to completely cure K. pneumoniae infection in the burn wound model. Maximum decrease in bacterial counts in different organs was observed at 72 h post infection. Histopathological examination of skin of phage-treated mice showed complete recovery of burn infection. Kpn5 phage was found to be highly effective among all the phages and equally effective when compared with a cocktail of all the phages. From these results, it can be concluded that phage therapy may have the potential to be used as stand-alone therapy for K. pneumoniae induced burn wound infection, especially in situations where multiple antibiotic-resistant organisms are encountered.