Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myeloid differentiation.
Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myeloid differentiation.
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DOI：
10.1182/blood.v73.1.131.131
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发表时间：
1989
期刊：
影响因子：
20.3
通讯作者：
AG Rosmarin;S. Weil;GL Rosner;J. Griffin;M. Arnaout;D. Tenen
中科院分区：
文献类型：
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作者：
AG Rosmarin;S. Weil;GL Rosner;J. Griffin;M. Arnaout;D. Tenen
在早期人髓系细胞向单核细胞和粒细胞分化的过程中，细胞粘附分子CD 11b/CD 18（Mo 1）的细胞表面表达显著增加，而杀菌酶髓过氧化物酶（MPO）的表达显著降低。以诱导型早幼粒细胞系HL-60为模型，我们研究了这些基因的mRNA表达。这些细胞沿着单核细胞和粒细胞途径的分化表明，CD 11 b/CD 18的两个亚基的mRNA水平在时间上和数量上以与该异二聚体的细胞表面表达的增加相似的模式增加。与此相反，MPO mRNA的表达下降的时间和定量模式类似的MPO蛋白在分化过程中已知的减少，这表明这些骨髓特异性蛋白的调节可能发生在mRNA表达水平。这些发现对于急性非淋巴细胞白血病分化阻滞的性质和髓系基因表达的调控具有重要意义。
During the course of differentiation of early human myeloid cells toward monocytes and granulocytes, cell surface expression of the cell adhesion molecule, CD11b/CD18 (Mo1) increases dramatically and expression of myeloperoxidase (MPO), a bacteriocidal enzyme, decreases markedly. Using the inducible promyelocytic cell line HL-60 as a model, we studied the mRNA expression of these genes. Differentiation of these cells along both a monocytic and a granulocytic pathway demonstrated that the mRNA levels of the two subunits of CD11b/CD18 increased in a pattern temporally and quantitatively similar to the increase in cell surface expression of this heterodimer. In contrast, the expression of MPO mRNA decreased in a temporal and quantitative pattern similar to the known decrease in MPO protein during differentiation, suggesting that regulation of these myeloid-specific proteins may occur at the level of mRNA expression. These findings have important implications with regard to the nature of the block in differentiation in acute nonlymphocytic leukemia and the regulation of myeloid gene expression.