Profiling and Compositional Analysis of the Exoproteome of Synechocystis Sp . PCC 6803
Profiling and Compositional Analysis of the Exoproteome of Synechocystis Sp . PCC 6803
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DOI：
10.13188/2329-1583.1000004
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Liyan Gao;Xiahe Huang;Haitao Ge;Yuanya;Zhang;Yu Kang;Longfa Fang;Kehui Liu;Yingchun Wang
中科院分区：
文献类型：
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作者：
Liyan Gao;Xiahe Huang;Haitao Ge;Yuanya;Zhang;Yu Kang;Longfa Fang;Kehui Liu;Yingchun Wang
外蛋白质组是单细胞蓝细菌集胞藻PCC 6803的细胞外环境的重要组成部分之一，集胞藻PCC 6803是一种光合模式生物，正在成为生产清洁和可再生生物燃料的潜在细胞工厂。在此，我们使用高分辨率质谱鉴定了该生物体的胞外蛋白质组，并分析了所鉴定的胞外蛋白质的来源。总共鉴定了201种蛋白质，包括细胞表面蛋白HlyA和周质蛋白FutA2，它们是外蛋白质组中最丰富的。这两种蛋白质的总量估计占细胞外环境中蛋白质总量的50%以上。超过100种蛋白质被预测为分泌性的或先前被鉴定为周质蛋白，并且这些蛋白质的总量是所有鉴定的胞外蛋白质的总量的近88%，确立分泌为胞外蛋白质组的蛋白质群体的主要贡献者。此外，发现2种蛋白质被严重糖基化，表明外蛋白质组是糖蛋白的某个子集的富集池。本研究为集胞藻外蛋白质组的研究提供了一个完整的目录，为相关领域的研究提供了宝贵的资源。
The exoproteome is one of the important components of the extracellular milieu of the unicellular cyanobacterium Synechocystis sp. PCC 6803, a photosynthetic model organism that is emerging as a potential cell factory for producing clean and renewable biofuel. Herein, we identified the exoproteome of this organism using high resolution mass spectrometry and analyzed the sources of origins of the identified extracellular proteins. In total, 201 proteins were identified, including the cell surface protein HlyA and the periplasmic protein FutA2 that are the most abundant in the exoproteome. The combined amount of the two proteins is estimated to constitute more than 50 percent of the total amount of proteins in the extracellular milieu. More than 100 proteins were predicted to be secretory or previously identified as periplasmic proteins, and the total amount of these proteins is nearly 88% of that of all identified extracellular proteins, establishing secretion as the major contributor to the protein population of the exoproteome. In addition, 2 proteins were found to be heavily glycosylated, suggesting that the exoproteome is an enriched pool of a certain subset of glycoproteins. Together, our work provided a comprehensive catalog of the Synechocystis exoproteome and a high valuable resource for the related research fields.