Estrogen-induced 24K protein in MCF-7 breast cancer cells is localized in granules.
Estrogen-induced 24K protein in MCF-7 breast cancer cells is localized in granules.
复制标题
MCF-7 乳腺癌细胞中雌激素诱导的 24K 蛋白位于颗粒中。
DOI：
10.1007/bf01806037
复制
发表时间：
1984
影响因子：
3.8
通讯作者：
McGuire,WL
中科院分区：
文献类型：
--
作者：
Ciocca,DR;Adams,DJ;Edwards,DP;Bjercke,RJ;McGuire,WL
我们以前曾报道过单克隆抗体的生产，检测，通过免疫组织化学，雌激素诱导的蛋白质的分子量为24，000道尔顿（24 K）。这种功能未知的蛋白质已在以下细胞中检测到：a）雌激素受体阳性乳腺癌细胞系，但在受体阴性细胞系中未检测到;和c）某些人类癌，包括乳腺肿瘤。为了检测这种24 K雌激素诱导蛋白的亚细胞定位，我们用体外生长的人乳腺癌细胞系（MCF-7）和裸鼠体内进行了光镜和电镜水平的免疫组织化学研究。7细胞在裸鼠腹水中生长，石蜡切片显示出明确的极性，24 K蛋白定位于细胞的顶端细胞质中。体外培养的MCF-7细胞经离心后，24 K蛋白主要分布于胞浆大颗粒中。免疫电镜观察发现，体外和体内生长的MCF-7细胞胞浆内均可见24 K蛋白颗粒，颗粒大小、形状和24 K免疫染色强度不同。形态学证据表明，24 K雌激素诱导蛋白是由细胞分泌的。
We have previously reported the production of monoclonal antibodies which detect, by immunohistochemistry, an estrogen-induced protein of molecular weight 24,000 daltons (24K). This protein, of unknown function, has been detected in: a) estrogen receptor-positive breast cancer cell lines but not in receptornegative lines; b) several human normal estrogen target organs; and c) certain human carcinomas, including breast tumors.To examine the subcellular localization of this 24K estrogen-induced protein, we have done immunohistochemical studies at light and electron microscopic levels using a human breast tumor cell line (MCF-7) grownin vitroand also in nude micein vivo.MCF-7 cells grown in the ascites fluid of nude mice and processed for paraffin sections showed a defined polarity, and the 24K protein was localized in the apical cytoplasm of the cells. After cytocentrifugation, MCF-7 cells grownin vitrodisplayed 24K protein mainly confined to large cytoplasmic granules. The presence of 24K protein in cytoplasmic granules was also seen by immunoelectronmicroscopy in MCF-7 cells grown bothin vitroandin vivo.The granules had different sizes, shapes, and 24K immunostaining intensity. The morphological evidence suggests that the 24K estrogeninduced protein is secreted from the cells.