Soil microbial abundance and community structure vary with altitude and season in the coniferous forests, China
Soil microbial abundance and community structure vary with altitude and season in the coniferous forests, China
复制标题
中国针叶林土壤微生物丰度和群落结构随海拔和季节变化
DOI：
10.1007/s11368-016-1593-0
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发表时间：
2017
影响因子：
3.6
通讯作者：
Yang WQ
中科院分区：
文献类型：
--
作者：
Zhang Li;Yang Wanqin;Xu Zhenfeng;Wu Fuzhong;Tan Bo;Liu Yang;Chen Lianghua;Wang Ao;Yang WQ
土壤微生物控制着森林生态系统中生物元素的循环和能量转化，对环境变化敏感。到目前为止，海拔和季节对土壤微生物的影响仍然未知。在3023、3298和3582 m的海拔梯度上选择沿着560 m的垂直过渡带，研究季节冻融对土壤微生物群落的潜在影响。解冻后期（TPL）。结果与讨论土壤细菌、古细菌和真菌的核糖体DNA（rDNA）拷贝数在GS至冻结期（FP和FPD）逐渐减少，在解冻期（TP和TPL）逐渐增加。微生物群落的多样性也随季节性冻融过程而变化。细菌和古细菌群落多样性指数（H）从GS到FP依次降低，然后再到TPL依次升高。Fungal communityHindex increased in the freezingprocess.ConclusionsOur studies suggests，西藏针叶林土壤微生物群落的丰度和结构随季节而变化，细菌和古菌群落相对于真菌群落对季节性冻融过程的响应更迅速。这可能对高山森林生态系统的碳和养分循环产生重要影响。因此，未来变暖引起的季节性冻融模式的变化将通过改变土壤微生物特性来影响土壤养分循环。
PurposeSoil microbes control the bioelement cycles and energy transformation in forest ecosystems, and are sensitive to environmental change. As yet, the effects of altitude and season on soil microbes remain unknown. A 560 m vertical transitional zone was selected along an altitude gradient from 3023, 3298 and 3582 m, to determine the potential effects of seasonal freeze-thaw on soil microbial community.Materials and methodsSoil samples were collected from the three elevations in the growing season (GS), onset of freezing period (FP), deeply frozen period (FPD), thawing period (TP), and later thawing period (TPL), respectively. Real-time qPCR and polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) were used to measure the abundance and structure of soil microbial community.Results and discussionThe bacterial, archaeal, and fungal ribosomal DNA (rDNA) copy numbers decreased from GS to freezing stage (FP and FPD) and then increased in thawing stage (TP and TPL). Similarly, the diversity of microbial community varied with seasonal freeze-thaw processes. The diversity index (H) of the bacterial and archaeal communities decreased from GS to FP and then increased to TPL. The fungal communityHindex increased in the freezing process.ConclusionsOur results suggested that abundance and structure of soil microbial community in the Tibetan coniferous forests varied by season and bacterial and archaeal communities respond more promptly to seasonal freeze-thaw processes relative to fungal community. This may have important implications for carbon and nutrient cycles in alpine forest ecosystems. Accordingly, future warming-induced changes in seasonal freeze-thaw patterns would affect soil nutrient cycles via altering soil microbial properties.