Tissue tectonics: morphogenetic strain rates, cell shape change and intercalation
Tissue tectonics: morphogenetic strain rates, cell shape change and intercalation
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DOI：
10.1038/nmeth.1327
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发表时间：
2009-06-01
期刊：
影响因子：
48
通讯作者：
Adams, Richard J.
中科院分区：
文献类型：
--
作者：
Blanchard, Guy B.;Kabla, Alexandre J.;Adams, Richard J.
形态发生过程中组织的动态重塑是个体细胞行为和集体细胞重排相结合的结果。然而，缺乏明确测量细胞行为并将其与组织形态发生联系起来的综合框架。在这里，我们介绍了这样一个运动学框架，在细胞簇或域的中间、介观水平上桥接细胞和组织行为。通过根据细胞位置的相对运动及其形状的演变来测量域变形，我们表征了测量细胞行为的基本类别的基本不变量，即细胞形状变化和细胞插层的张量速率。在此过程中，我们引入了细胞嵌入作为连续过程的明确定义。我们在组织形态发生的三种模型中绘制了时空应变率图，深入了解形态发生机制。我们的定量方法对于形态发生表型的精确表征和比较具有广泛的相关性。
The dynamic reshaping of tissues during morphogenesis results from a combination of individual cell behaviors and collective cell rearrangements. However, a comprehensive framework to unambiguously measure and link cell behavior to tissue morphogenesis is lacking. Here we introduce such a kinematic framework, bridging cell and tissue behaviors at an intermediate, mesoscopic, level of cell clusters or domains. By measuring domain deformation in terms of the relative motion of cell positions and the evolution of their shapes, we characterized the basic invariant quantities that measure fundamental classes of cell behavior, namely tensorial rates of cell shape change and cell intercalation. In doing so we introduce an explicit definition of cell intercalation as a continuous process. We mapped strain rates spatiotemporally in three models of tissue morphogenesis, gaining insight into morphogenetic mechanisms. Our quantitative approach has broad relevance for the precise characterization and comparison of morphogenetic phenotypes.