Hyper-Rayleigh scattering of protein-modified gold nanoparticles
Hyper-Rayleigh scattering of protein-modified gold nanoparticles
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DOI：
10.1016/s0003-2697(03)00353-1
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发表时间：
2003-09-01
影响因子：
2.9
通讯作者：
Lu, ZH
中科院分区：
文献类型：
--
作者：
Zhang, CX;Zhang, Y;Lu, ZH
利用超瑞利散射（HRS）技术研究了蛋白质修饰金纳米粒子的非线性光学性质。当用波长为1064 nm的激光脉冲泵浦时，已经获得了来自涂覆有山羊抗人IgG的纳米颗粒的HRS信号。含IgG的金纳米颗粒的HRS信号比裸金纳米颗粒的HRS信号大。这可以用非中心对称效应来解释。还发现，当加入抗原时，由于金纳米颗粒聚集，来自IgG包被的金纳米颗粒的HRS信号可以大大增加。我们的实验发现，HRS方法可以产生一个可测量的信号与10 μ g/ml的抗原添加，而使用紫外光谱检测的比色法需要100 μ g/ml的抗原添加。结果表明，免疫金纳米颗粒的HRS测量可能成为一种潜在的免疫测定法，用于测定水溶液样品中的小水平抗原。(C)2003 Elsevier Science（美国）。All rights reserved.
The nonlinear optical properties of protein-modified gold nanoparticles has been studied by the hyper-Rayleigh scattering (HRS) technique. HRS signals from the nanoparticles coated with goat-anti-human IgG have been obtained when pumped with a laser pulse with a wavelength of 1064 nm. The HRS signals of gold nanoparticles with IgG were larger than those of bare gold nanoparticles. This can be explained by a noncentrosymmetric effect. It was also found that the HRS signals from the IgG-coated gold nanoparticles could be greatly increased when the antigen was added due to gold nanoparticle aggregation. Our experiment found that the HRS method could produce a measurable signal with 10 mug/ml antigen added, while the colorimetric method using UV spectrum detection required 100 mug/ml of added antigen. The results show that the HRS measurement of immunogold nanoparticles could become a potential immunoassay in determining small levels of antigen in aqueous samples. (C) 2003 Elsevier Science (USA). All rights reserved.