Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia
Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia
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DOI：
10.1002/jnr.20604
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发表时间：
2005-09-15
影响因子：
4.2
通讯作者：
Okano, H
中科院分区：
文献类型：
--
作者：
Tonchev, AB;Yamashima, T;Okano, H
成年啮齿动物脑缺血可增加沿侧脑室前角的室下区内源性神经祖细胞的增殖，并诱导脑缺血后纹状体和皮层的神经发生。成年灵长类动物的大脑是否保留了对缺血性损伤的类似反应能力尚不确定。我们使用DNA合成指示剂溴脱氧尿苷（BrdU）标记成年猕猴的新生细胞，结果显示，在短暂性全脑缺血20分钟后的第二周，SVZa中具有祖细胞表型（BrdU和标记物Musashi1、Nestin和β iii -微管蛋白双阳性）的细胞增殖增加。随后的祖细胞迁移似乎仅限于向嗅球的吻侧迁移流，缺血增加了成体产生的细胞在具有祖细胞表型的SVZa中保留其位置的比例。尽管缺乏证据表明祖细胞向脑缺血后纹状体或前额叶新皮层迁移，但一小部分brdu标记的细胞持续表达有丝分裂后神经元的特征（NeuN蛋白和转录因子阳性）。Tbr1和Islet1)在缺血后至少79天内在这两个区域表达。综上所述，我们的数据表明，在脑缺血损伤后，成年灵长类动物端脑的神经源性反应增强。(c) 2005 Wiley-Liss, Inc。
Cerebral ischemia in adult rodent models increases the proliferation of endogenous neural progenitor cells residing in the subventricular zone along the anterior horn of the lateral ventricle (SVZa) and induces neurogenesis in the postischemic striatum and cortex. Whether the adult primate brain preserves a similar ability in response to an ischemic insult is uncertain. We used the DNA synthesis indicator bromodeoxyuridine (BrdU) to label newly generated cells in adult macaque monkeys and show here that the proliferation of cells with a progenitor phenotype (double positive for BrdU and the markers Musashi1, Nestin, and beta III-tubulin) in SVZa increased during the second week after a 20-min transient global brain ischemia. Subsequent progenitor migration seemed restricted to the rostral migratory stream toward the olfactory bulb and ischemia increased the proportion of adult-generated cells retaining their location in SVZa with a progenitor phenotype. Despite the lack of evidence for progenitor cell migration toward the postischemic striatum or prefrontal neocortex, a small but sustained proportion of BrdU-Iabeled cells expressed features of postmitotic neurons (positive for the protein NeuN and the transcription factors. Tbr1, and Islet1) in these two regions for at least 79 days after ischemia. Taken together, our data suggest an enhanced neurogenic response in the adult primate telencephalon after a cerebral ischemic insult. (c) 2005 Wiley-Liss, Inc.