Regulation of Nogo-B expression in the lesion of aortic aneurysms
Regulation of Nogo-B expression in the lesion of aortic aneurysms
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DOI：
10.1111/j.1440-1681.2007.04673.x
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发表时间：
2007-09-01
影响因子：
2.9
通讯作者：
Rui, Yao-cheng
中科院分区：
文献类型：
--
作者：
Pan, Jing-Wei;Wei, Meng;Rui, Yao-cheng
我们之前的研究表明，溶血卵碱诱导Nogo-B在内皮细胞中高表达，并在内皮细胞中下调，从而导致动脉粥样硬化病变。然而，Nogo-B在主动脉瘤发展中的作用尚不清楚。在本研究中，我们从31例接受移植手术的患者身上获得了胸主动脉瘤（TAA）和邻近无动脉瘤改变的正常胸主动脉组织（NTA）。采用半定量逆转录聚合酶链反应、western blotting和免疫组织化学检测Nogo-B mRNA和蛋白表达水平。结果表明，与NTA病变相比，TAA病变中Nogo-B mRNA表达水平下降至45%，TAA中蛋白表达水平下降至35%。主动脉标本组织Nogo免疫组化染色提示Nogo参与新生血管和平滑肌细胞增殖。TAA病变内皮细胞褐色染色较弱，提示Nogo-B在TAA病变中表达较低。这些结果表明Nogo-B mRNA和蛋白在TAA病变中表达下调。由此可见，TAA病变中Nogo-B蛋白表达的降低与动脉瘤的形成密切相关，Nogo-B可能在动脉瘤的病理过程中起到保护作用。
Our previous study showed that Nogo-B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo-B in the development of aortic aneurysms remains unclear.In the present study, segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues (NTA) without aneurysmal changes were obtained from 31 patients undergoing graft surgery. The mRNA and protein expression levels of Nogo-B were measured with semiquantitative reverse transcription-polymerase chain reaction, western blotting and immunohistochemistry.The results demonstrate that Nogo-B mRNA expression levels in TAA lesions decreased to 45% compared with levels in NTA lesions and that protein levels in TAA decreased to 35%. Tissue Nogo immunohistochemical staining in aortic specimens suggested the involvement of Nogo in neovascularization and smooth muscle cell proliferation. The weaker brown staining of endothelial cells in TAA lesions suggested the lower expression of Nogo-B in TAA lesions.These results demonstrate that Nogo-B mRNA and protein expression are downregulated in TAA lesions. It is concluded that the reduction of Nogo-B protein expression in TAA lesions is closely correlated to the formation of aneurysm and that Nogo-B may play a protective role in the pathological process of aneurysms.