Fungal Dysbiosis in Mucosa-associated Microbiota of Crohn's Disease Patients
Fungal Dysbiosis in Mucosa-associated Microbiota of Crohn's Disease Patients
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DOI：
10.1093/ecco-jcc/jjv209
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发表时间：
2016-03-01
影响因子：
8
通讯作者：
Sokol, Harry
中科院分区：
文献类型：
--
作者：
Liguori, Giuseppina;Lamas, Bruno;Sokol, Harry
背景和目标：肠道微生物群参与许多生理功能，其失衡与多种疾病有关，特别是炎症性肠病。粘膜相关微生物群可能在诱导宿主免疫和炎症过程中起关键作用。虽然真菌在炎症性疾病发病机制中的作用已被提出，但真菌微生物群尚未被深入研究。在这里，我们分析了克罗恩病患者和健康subjects.Methods的粘膜相关的微生物群的细菌和真菌的组成：我们的前瞻性，观察性研究评估了23克罗恩病患者[16在耀斑，7在缓解]和10名健康受试者的粘膜相关的微生物群的细菌和真菌的组成，分别使用16S [MiSeq]和ITS2 [焦磷酸测序]测序。通过真实的定量聚合酶链反应评估全球真菌负荷。结果：克罗恩病患者的细菌微生物群的特征是生物多样性受到限制。变形菌门和梭菌门数量增加。与健康受试者相比，克罗恩病发作中的总体真菌负荷显著增加[p < 0.05]。在这两组中，结肠粘膜相关的真菌微生物群以担子菌门和子囊菌门为主。Cystofilobasidiaceae科和光滑念珠菌属在克罗恩病中的比例过高。酿酒酵母和Filobasidium uniguttulatum物种与非发炎粘膜，而Xylariales订单与发炎mucosa.Conclusions：我们的研究证实了改变的细菌菌群，是第一个证明存在的改变的真菌菌群在克罗恩病患者，这表明真菌可能在发病机制中发挥作用。
Background and Aims: Gut microbiota is involved in many physiological functions and its imbalance is associated with several diseases, particularly with inflammatory bowel diseases. Mucosa-associated microbiota could have a key role in induction of host immunity and in inflammatory process. Although the role of fungi has been suggested in inflammatory disease pathogenesis, the fungal microbiota has not yet been deeply explored. Here we analysed the bacterial and fungal composition of the mucosa-associated microbiota of Crohn's disease patients and healthy subjects.Methods: Our prospective, observational study evaluated bacterial and fungal composition of mucosa-associated microbiota of 23 Crohn's disease patients [16 in flare, 7 in remission] and 10 healthy subjects, using 16S [MiSeq] and ITS2 [pyrosequencing] sequencing, respectively. Global fungal load was assessed by real time quantitative polymerase chain reaction.Results: Bacterial microbiota in Crohn's disease patients was characterised by a restriction in biodiversity. with an increase of Proteobacteria and Fusobacteria. Global fungus load was significantly increased in Crohn's disease flare compared with healthy subjects [p < 0.05]. In both groups, the colonic mucosa-associated fungal microbiota was dominated by Basidiomycota and Ascomycota phyla. Cystofilobasidiaceae family and Candida glabrata species were overrepresented in Crohn's disease. Saccharomyces cerevisiae and Filobasidium uniguttulatum species were associated with non-inflamed mucosa, whereas Xylariales order was associated with inflamed mucosa.Conclusions: Our study confirms the alteration of the bacterial microbiota and is the first demonstration of the existence of an altered fungal microbiota in Crohn's disease patients, suggesting that fungi may play a role in pathogenesis.