Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea.
Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea.
复制标题
间苯二酚的鉴定和纯化，间苯二酚是阿波班（腌制和厌氧发酵）茶特有的抗氧化剂。
DOI：
10.1016/j.foodres.2013.05.036
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发表时间：
2013
影响因子：
8.1
通讯作者：
Kuzuhara T.
中科院分区：
文献类型：
--
作者：
Hiasa M;Kurokawa M;Ohta K;Esumi T;Akita H;Niki K;Yagi Y;Echigo N;Hatakeyama D;Kuzuhara T.
阿波班茶（阿波，德岛县在日本;班，“晚上”在日本）是一种茶专用于德岛地区。阿瓦班茶叶的独特之处在于它们是通过腌制和厌氧发酵生产的。在这里，我们报告的识别和纯化的抗氧化剂，是专门存在于阿波班茶，但不存在于绿色和红茶。该分子被鉴定为间苯二酚，被发现在体外表现出自由基清除活性，其水平相当于绿色茶中的（-）-表没食子儿茶素没食子酸酯。荧光显微镜和流式细胞术结果进一步表明，间苯二酚以浓度依赖性方式降低HEK 293 T细胞、人脐静脉内皮细胞和Jurkat细胞中活性氧的水平。此外，3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物测定表明用10 mM间苯二酚处理不影响细胞活力。我们的研究结果表明，阿瓦班茶的性质是相当不同的那些绿色和红茶，因此是一个独特的抗氧化饮料。我们还建议，间苯二酚是一种新的茶基抗氧化剂。
Awa-ban tea (Awa, Tokushima prefecture in Japan;ban, “evening” in Japanese) is a type of tea exclusive to the Tokushima area. Awa-ban tea leaves are unique in that they are produced via pickling and anaerobic fermentation. Here, we report the identification and purification of an antioxidant that is specifically present in Awa-ban tea but is absent in green and black tea. The molecule, identified as resorcinol, was found to exhibit radical-scavenging activity in vitro at levels equivalent to those of (–)-epigallocatechin gallate in green tea. Fluorescence microscopy and flow cytometry results further showed that resorcinol decreased the levels of reactive oxygen species in HEK293T cells, human umbilical vein endothelial cells, and Jurkat cells in a concentration-dependent manner. Moreover, the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated that treatment with 10 mM resorcinol did not affect cell viability. Our results indicate that Awa-ban tea has properties that are quite distinct from those of green and black tea and is therefore a unique antioxidant beverage. We also suggest that resorcinol is a novel tea-based antioxidant.