Exploring the Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in Silico and Biochemical Analyses.
Exploring the Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in Silico and Biochemical Analyses.
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通过计算机模拟和生化分析探索参与脂多糖生物合成的细胞质膜蛋白的拓扑结构。
DOI：
10.1007/978-1-0716-2581-1_5
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Monjarás Feria J
中科院分区：
文献类型：
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作者：
Monjarás Feria J
在缺乏三维结构的情况下，揭示膜蛋白的拓扑结构可以提供相关信息来识别跨膜螺旋的数量和方向以及关键氨基酸残基的定位，从而有助于更好地理解功能和分子间关联。拓扑结构可以通过生物信息学分析进行计算机预测或通过生化方法求解。在本章中，我们描述了采用生物信息学方法预测膜蛋白拓扑的管道，然后通过取代半胱氨酸可及性方法进行实验验证并分析蛋白质的寡聚状态。
In the absence of a tri-dimensional structure, revealing the topology of a membrane protein provides relevant information to identify the number and orientation of transmembrane helices and the localization of critical amino acid residues, contributing to a better understanding of function and intermolecular associations. Topology can be predicted in silico by bioinformatic analysis or solved by biochemical methods. In this chapter, we describe a pipeline employing bioinformatic approaches for the prediction of membrane protein topology, followed by experimental validation through the substituted-cysteine accessibility method and the analysis of the protein’s oligomerization state.