Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche.
Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche.
复制标题
肌肉干细胞生态位的离体可视化和分析。
DOI：
10.1007/7651_2018_177
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Krauss,RobertS
中科院分区：
文献类型：
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作者：
Goel,AvivaJ;Krauss,RobertS
成人骨骼肌干细胞被称为卫星细胞，对于组织损伤后的肌肉再生是必不可少的。卫星细胞位于肌肉纤维及其周围的基底板之间的特殊微环境中。这个局部的小生境作为一个隔间来保护卫星细胞的功能，并提供促进对伤害的快速反应的信号。这种局部生态位的可视化使这种生态位衍生信号的阐明成为可能。在这里，我们描述了分离单个肌纤维及其相关卫星细胞的技术，用于体外可视化和分析完整的肌肉干细胞生态位。
Adult skeletal muscle stem cells, termed satellite cells, are essential for regenerating muscle after tissue damage. Satellite cells are located in a specialized microenvironment between muscle fibers and their surrounding basal lamina. This local niche serves as a compartment to preserve satellite cell function and provides signals that facilitate the rapid response to injury. Visualization of this local niche enables the elucidation of such niche-derived signals. Here, we describe techniques for isolating single myofibers with their associated satellite cells for ex vivo visualization and analysis of an intact muscle stem cell niche.