Localization of the CAPRICE-ENHANCER OF TRY AND CPC1 chimera protein in Arabidopsis root epidermis
Localization of the CAPRICE-ENHANCER OF TRY AND CPC1 chimera protein in Arabidopsis root epidermis
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DOI：
10.1080/09168451.2017.1343120
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
R. Tominaga-Wada;T. Kurata;T. Wada
中科院分区：
文献类型：
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作者：
R. Tominaga-Wada;T. Kurata;T. Wada
卡普赖斯(CPC)编码一种R3型MYB转录因子，促进根毛分化。在此之前，我们已经证明了在拟南芥中，CPC蛋白从无毛细胞移动到邻近细胞并诱导根-毛分化。此外，我们在CPC中提出了两个细胞间运动信号序列：S1和S2。然而，S1：2xGFP：S2嵌合体蛋白不能在根表皮细胞之间移动。在这里，我们证明了S1和S2序列不赋予GFP蛋白细胞间移动或核定位能力。Try和CPC1(ETC1)基因的增强子编码CPC同系物R3MYB；该蛋白不具有细胞间运动能力或S1序列。构建了CPCP：S1：ETC1：2xGFP，并将其导入拟南芥中，以阐明S1序列是否能诱导ETC1细胞间的运动能力。我们的结果表明，添加S1序列不足以使ETC1获得细胞间的运动能力。
The CAPRICE (CPC) encodes an R3-type MYB transcription factor, which promotes root-hair differentiation. Previously, we showed that the CPC protein moves from the non-hair cell to the neighboring cell and induces root-hair differentiation in Arabidopsis. In addition, we proposed two cell-to-cell movement signal sequences, S1 and S2, in CPC. However, an S1:2xGFP:S2 chimera protein did not move between root epidermal cells. Here, we show that the S1 and S2 sequences do not confer cell-to-cell movement or nuclear localization ability to a GFP protein. The ENHANCER OF TRY AND CPC1 (ETC1) gene encodes the CPC homolog R3 MYB; this protein does not possess cell-to-cell movement ability or the S1 sequence. To elucidate whether the S1 sequence can induce cell-to-cell movement ability in ETC1, CPCp:S1:ETC1:2xGFP was constructed and introduced into Arabidopsis. Our results indicate that the addition of the S1 sequence was not sufficient for ETC1 to acquire cell-to-cell movement ability.