Hybrid phase ligation for efficient synthesis of histone proteins.
Hybrid phase ligation for efficient synthesis of histone proteins.
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DOI：
10.1039/c5ob02195b
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发表时间：
2016-03-07
影响因子：
3.2
通讯作者：
Ottesen JJ
中科院分区：
文献类型：
--
作者：
Yu RR;Mahto SK;Justus K;Alexander MM;Howard CJ;Ottesen JJ
我们介绍了一种混合固-液相连接方法，该方法结合了固相连接和液相连接在修饰组蛋白全合成中的效率。两个接头策略允许在固相上的整个工作中进行分析，并通过在外部Rink处切割使产率最大化，而内部HMBA接头允许任何蛋白质序列的天然羧基末端。我们证明了两种组蛋白的这种方法：三乙酰化H4-K5 ac，K12 ac，K91 ac和CENP-A-K124 ac。
We introduce a hybrid solid-solution phase ligation approach that combines the efficiency of solid phase ligation with solution phase ligation in the total synthesis of modified histone proteins. A two linker strategy allows analysis throughout work on the solid phase and maximizes yields through cleavage at an external Rink, while an internal HMBA linker allows the native carboxyl terminus for any protein sequence. We demonstrate this approach for two histone proteins: triple-acetylated H4-K5ac,K12ac,K91ac and CENP-A-K124ac.