Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism
Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism
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DOI：
10.1016/j.ijfoodmicro.2010.04.025
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发表时间：
2010-06-30
影响因子：
5.4
通讯作者：
Fu, Tzu-Fun
中科院分区：
文献类型：
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作者：
Kao, Tseng-Ting;Tu, Hung-Chi;Fu, Tzu-Fun
金黄色葡萄球菌(S.金黄色葡萄球菌）是引起全世界传染病和食源性疾病的最常见病原体之一。寻找药物和化学化合物对抗这种细菌的需求仍然存在。葡萄籽提取物是一种富含多酚类物质的天然食品，它能抑制S.金黄色。此外，还测定了S.当培养基中存在GSE时，金黄色葡萄球菌的生长速度显著降低。通过向培养基中加入THE、5，10-亚甲基四氢叶酸或甲硫氨酸，可逆转GSE诱导的生长抑制。胸腺嘧啶核苷和甲硫氨酸诱导的不同的拯救效应表明，GSE诱导的叶酸介导的一碳代谢紊乱对甲硫氨酸循环的影响比对一磷酸胸腺嘧啶核苷（TMP）合成的影响更深远。在感染S.（用GSE预孵育弗伦斯。因此，GSE可作为一种有效的天然替代品，用于控制S.并采取适当的安全措施。(C)2010爱思唯尔有限公司版权所有。
Staphylococcus aureus (S. aureus) is one of the most common pathogens that causes infectious and foodborne diseases worldwide. Searching for drug and chemical compounds against this bacterium is still in demand. We found that grape seed extract (GSE), a natural food product rich in polyphenols, inhibited the dihydrofolate reductase activity and growth of S. aureus. In addition, the intracellular content of tetrahydrofolate (THF), the major folate species identified in S. aureus, was significantly decreased when GSE was present in medium. The GSE-induced growth inhibition was reversed by adding, THE, 5,10-methylenetetrahydrofolate or methionine to the medium. The differential rescuing effects elicited by thymidine and methionine indicated that GSE-induced perturbation in folate-mediated one-carbon metabolism has more profound impact on methionine cycle than on thymidine monophosphate (TMP) synthesis. Significantly reduced inflammatory responses and mortality were observed in zebrafish infected with S. (wrens pre-incubated with GSE. We conclude that GSE might serve as an effective natural alternative for the control of food poisoning caused by S. aureus with proper safety measure. (C) 2010 Elsevier B.V. All rights reserved.