SELECTIVE LOCALIZATION OF THE PARATHYROID SECRETORY PROTEIN-I ADRENAL-MEDULLA CHROMOGRANIN-A PROTEIN FAMILY IN A WIDE VARIETY OF ENDOCRINE-CELLS OF THE RAT
SELECTIVE LOCALIZATION OF THE PARATHYROID SECRETORY PROTEIN-I ADRENAL-MEDULLA CHROMOGRANIN-A PROTEIN FAMILY IN A WIDE VARIETY OF ENDOCRINE-CELLS OF THE RAT
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DOI：
10.1210/endo-114-6-1963
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发表时间：
1984-01-01
期刊：
影响因子：
4.8
通讯作者：
ELDE, R
中科院分区：
文献类型：
--
作者：
COHN, DV;ELTING, JJ;ELDE, R
甲状旁腺分泌蛋白-I(SP-I)和肾上腺髓质嗜铬细胞的软骨颗粒蛋白A(CGA)即使不完全相同，也具有相似的化学结构。这两种蛋白质都包含在分泌颗粒中，并且似乎与颗粒含量有关，例如，在甲状旁腺中含有甲状旁腺素[甲状旁腺激素]，在肾上腺中含有肾上腺素和多巴胺β-羟基酶。用牛SP-I和猪CGA的抗血清以及多种多肽激素的抗血清对大鼠组织进行免疫荧光研究，以确定这些蛋白的可能分布和细胞定位。除了先前证实的在甲状旁腺和肾上腺细胞中的存在外，SP-I/CGA蛋白家族还在含有降钙素的甲状腺细胞中被检测到，通常在SRIF中但不在甲状腺球蛋白中被检测到；在垂体前叶的细胞中被检测到TSH/FSH/LH[黄体生成素]的α-亚单位，但在GH[生长激素]、PRL[催乳素]、ACTH或β-内啡肽的细胞中未被检测到；在胰岛细胞中被检测到SRIF[生长抑素]和胰腺多肽相关的多肽，但不被胰岛素或胰升糖素所检测；在一些含有SRIF的细胞中的腹膜和肠系膜神经节中被检测到；胃窦内含SRIF的细胞，但不含胃泌素。SP-I/CgA在肝、肾、腮腺和腺泡胰腺的细胞以及它们的垂体中叶和后叶均未检测到。显然，这个蛋白家族在大鼠的许多不同的内分泌肽细胞中分布广泛，但受到高度限制，其中许多被认为是APUD[胺前体摄取和脱羧基]细胞系列。提示SP-I/CGA可能在分泌过程中发挥一定的生理作用，也可能在分泌后在外周发挥自身的作用。
Secretory protein-I (SP-I) of parathyroid glands and chomogranin A (CGA) of adrenal medullary chromaffin cells are chemically similar if not identical proteins. Both proteins are contained within secretory granules and appear to be cosecreted with granule contents, for example, in the parathyroid with PTH [parathyroid hormone] and in the adrenal with epinephrine and dopamine .beta.-hydroxylase. Antisera to bovine SP-I and porcine CGA, together with antisera to a variety of peptide hormones, were used in an immunofluorescence study of rat tissues in order to determine the probable distribution and cellular localization of these proteins. In addition to their previously demonstrated presence in parathyroid and adrenal cells, the SP-I/CGA protein family was detected in cells of the thyroid that contained calcitonin and often SRIF but not thyroglobulin; in cells of the anterior pituitary staining for the .alpha.-subunit of TSH/FSH/LH [luteinizing hormone] but not in cells staining for GH [growth hormone], PRL [prolactin], ACTH or .beta.-endorphin; in pancreatic islet cells staining for SRIF [somatostatin] and pancreatic polypeptide-related peptides, but not for insulin or glucagon; in the celiac and mesenteric ganglia in cells some of which contained SRIF; and in the gastric antrum in cells containing SRIF, but not gastrin. SP-I/CGA was not detected in cells of the liver, kidney, parotid gland or acinar pancreas or in their intermediate or posterior lobes of the pituitary. Apparently this protein family enjoys a widespread but highly restricted distribution in many different endocrine-peptide cells of the rat, many that are believed to be of the APUD [amine precursor uptake and decarboxylation] cell series. The possibility is raised that SP-I/CGA plays some physiological role in the secretory process or exerts an effect of its own in the periphery after secretion.