EPITAXIAL-GROWTH OF PROTEIN CRYSTALS FROM 2-DIMENSIONAL CRYSTALS ON LIPID LAYERS

EPITAXIAL-GROWTH OF PROTEIN CRYSTALS FROM 2-DIMENSIONAL CRYSTALS ON LIPID LAYERS
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DOI:
10.1016/0959-440x(95)80056-5
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发表时间:
1995-10-01
影响因子:
6.8
通讯作者:
EDWARDS, AM
EDWARDS, AM
中科院分区:
生物学2区
文献类型:
--
作者:
DARST, SA;EDWARDS, AM

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在脂质层上形成的蛋白质的二维晶体有效地使三维蛋白质晶体的外延生长成核。适合于X射线晶体学的晶体可以以这种方式更快地生长,并且使用比使用常规方法时低得多的蛋白质和沉淀剂浓度。
Two-dimensional crystals of proteins formed on lipid layers effectively nucleate the epitaxial growth of three-dimensional protein crystals. Crystals suitable for X-ray crystallography can be grown in this way more rapidly, and using substantially lower concentrations of protein and precipitants, than when using conventional methods.