Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture.
Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture.
复制标题
从悬浮细胞培养物中去除细胞外弓形虫速殖子。
DOI：
10.1016/j.parint.2016.01.012
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Takashima Y.
中科院分区：
文献类型：
--
作者：
Baba M;Kitoh K;Takashima Y.
弓形虫的速殖子是一种专性的细胞内寄生虫，它主动入侵多种细胞类型。弓形虫感染白细胞，并传播到远处的器官，如脑、肺和肌肉。然而，这种机制经常出现。从感染的白细胞到外周器官的性腺病毒传播尚不清楚。为了显示体内感染的白细胞和细胞内寄生虫的动态，以前的研究已经准备了T。感染弓形虫的白细胞，并将它们注射到实验动物的循环中。然而，当感染的白细胞在体外制备时，一些胞外速殖子残留在白细胞培养中，因为这些胞外速殖子几乎不可能被洗掉。这些胞外速殖子可能会扭曲实验结果。在这项研究中，我们报告了一种使用抗体偶联磁珠从白细胞培养悬液中去除胞外速殖子的方法。用这种方法可以有效地消除悬浮细胞培养中的胞外速殖子。
Tachyzoites of Toxoplasma gondii, an obligate intracellular parasite, actively invade a broad spectrum of cell types.T. gondiiinfects leukocytes and spreads to distant organs such as the brain, lungs and muscles. However, the mechanism ofT. gondiitransmission from infected leukocytes to peripheral organs is unknown. To show the dynamics of infected leukocytes and intracellular parasitesin vivo, previous studies have preparedT. gondii-infected leukocytes and injected them into circulation in experimental animals. However, when the infected leukocytes are preparedin vitro, some extracellular tachyzoites remain in the leukocyte cell culture because it is almost impossible to wash out these extracellular tachyzoites. These extracellular tachyzoites may distort experimental results. In this study, we report a method for removing extracellular tachyzoites from leukocyte culture suspension using antibody-conjugated magnetic beads. Using this method, extracellular tachyzoites in suspension cell culture can be effectively eliminated.