Cytoskeletal mechanics of proplatelet maturation and platelet release.
Cytoskeletal mechanics of proplatelet maturation and platelet release.
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DOI：
10.1083/jcb.201006102
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发表时间：
2010-11-15
期刊：
影响因子：
--
通讯作者：
Italiano JE Jr
中科院分区：
文献类型：
--
作者：
Thon JN;Montalvo A;Patel-Hett S;Devine MT;Richardson JL;Ehrlicher A;Larson MK;Hoffmeister K;Hartwig JH;Italiano JE Jr
定义了原血小板延伸从巨核细胞转化为成熟血小板过程中的新步骤。巨核细胞通过将其细胞质重塑为长长的前血小板延伸部分来生成血小板，这些延长部分充当了生产血小板的装配线。尽管已经研究了原血小板延长的机制，但对原血小板成熟和释放的终末步骤仍知之甚少。为了阐明这一过程，分离了释放的前血小板，并评估了它们转化为单个血小板的情况。这使得我们能够(A)定义和量化血小板成熟的不同阶段，(B)确定血小板产生的新的中间阶段--前血小板，(C)描述前/原血小板相互转换所涉及的细胞骨架机制，以及(D)前血小板分裂和血小板释放的模型。前血小板是直径2-10微米的无核盘状颗粒，能够通过扭转微管作用力可逆地转化为细长的前血小板，这种作用力可以在表达绿色荧光蛋白-β1-微管蛋白的前血小板中可见。在培养过程中，随着较大的原血小板经历连续的分裂，并因剪切而增强，从原血小板末端释放的血小板以时间的增加速度发生。
New steps in the process of conversion of proplatelet extensions from megakaryocytes into mature platelets are defined. Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2–10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP–β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear.
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影响因子：
20.3
作者：
Lecine, P;Villeval, JL;Shivdasani, RA
通讯作者：
Shivdasani, RA
影响因子：
20.3
作者：
Richardson, JL;Shivdasani, RA;Italiano, JE
通讯作者：
Italiano, JE
影响因子：
20.3
作者：
Schulze, H;Korpal, M;Shivdasani, RA
通讯作者：
Shivdasani, RA
DOI：
10.1111/j.1538-7836.2009.03387.x
发表时间：
2009-07
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Italiano JE Jr;Battinelli EM
通讯作者：
Battinelli EM
影响因子：
20.3
作者：
Heijnen, HFG;Debili, N;Sixma, JJ
通讯作者：
Sixma, JJ