A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes
A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes
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DOI：
10.1007/s10815-010-9445-0
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Fukui, Yutaka
中科院分区：
文献类型：
--
作者：
Watanabe, Hiroyuki;Suzuki, Hiroshi;Fukui, Yutaka
为探讨小鼠卵母细胞体积对家畜精子染色体分析效率的影响，将牛、羊、猪和狗的精子头注入小鼠卵母细胞，然后与去核小鼠卵母细胞进行融合，结果表明，随着卵母细胞体积的增加，注入精子后形态正常的卵母细胞比例增加，从而提高了公牛的染色体检出率，公羊和狗的精子。受体卵母细胞的体积不影响染色体的完整性。此外，在公牛中，融合小鼠卵母细胞检测到的染色体完整性与同源系统中的相似。另一方面，尽管使用融合卵母细胞，猪精子的染色体平板不能被检测到。这些数据表明，融合小鼠卵母细胞提高了公牛，公羊和狗精子染色体检测的效率。
The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa.Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes.The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes.These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa.