A NOTE ON THE USE OF DINITROSALICYLIC ACID FOR DETERMINING THE PRODUCTS OF ENZYMATIC-REACTIONS
A NOTE ON THE USE OF DINITROSALICYLIC ACID FOR DETERMINING THE PRODUCTS OF ENZYMATIC-REACTIONS
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DOI：
10.1007/bf00269074
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发表时间：
1988-11-01
影响因子：
5
通讯作者：
BAILEY, MJ
中科院分区：
文献类型：
--
作者：
BAILEY, MJ
讨论了用二硝基水杨酸(DNS)检测反应产物的酶分析方法中的非线性因素。通常的做法是在定量还原化合物之前稀释反应产物，这是造成非线性的原因之一。在大多数现代版本的方法中，底物不足也是一个重要的促成因素，尽管原始程序被正确地设计为确保线性反应。强调了用暗示线性的单位(katals或International Units)表示非线性反应结果所涉及的固有不准确性。
Factors contributing to non-linearity of enzyme assays incorporating detection of reaction products using dinitrosalicylic acid (DNS) are discussed. The common practice of diluting reaction products before quantification of reducing compounds is shown to be one cause of non-linearity. Insufficiency of substrate is also an important contributory factor in most modern versions of the method, although the original proceudre was correctly designed to ensure a linear reaction. The inherent inaccuracy involved in expression of the results of non-linear reactions in units implying linearity (katals or International Units) is emphasized.