Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus
Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus
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DOI：
10.1016/j.aquaculture.2006.06.032
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发表时间：
2006-09-29
期刊：
影响因子：
4.5
通讯作者：
Dai, Wei
中科院分区：
文献类型：
--
作者：
Du, Huahua;Xu, Zirong;Dai, Wei
为了研究蛋白结构对WSSV囊膜蛋白保护作用的影响，我们在大肠杆菌(pET-VP28)和昆虫细胞(BmNPV-VP28)中表达了VP28蛋白。利用杆状病毒(BmNPV)表达系统获得正确折叠的VP28蛋白。克氏原虾肌肉注射重组pET-VP28和BmNPV-VP28感染细胞的裂解物，然后肌肉注射WSSV以评估保护时间。与注射pET-VP28的小龙虾相比，注射BmNPV-VP28的小龙虾的死亡率普遍较低，与注射空载体BmNPV和PET-30a的对照组相比，其相对存活率分别为92%和39%。这些结果表明，BMN细胞产生的VP28蛋白比在大肠杆菌中产生的VP28蛋白具有更好的保护作用。(C)2006爱思唯尔B.V.保留所有权利。
In order to investigate whether protein structure has an effect on protective effect of envelope protein of WSSV, VP28 protein was expressed both in Escherichia coli (pET-VP28) and insect (BmN) cells (BmNPV-VP28). The baculovirus (BmNPV) expression system was used to obtain correctly folded VP28 protein. Procambarus clarkii crayfish were intramuscularly injected with lysates of cells infected with recombinant pET-VP28 and BmNPV-VP28, respectively, and then challenged by intramuscular injection of WSSV to assess the duration of protection. The crayfish injected with BmNPV-VP28 showed generally lower mortality rates when compared to crayfish injected with pET-VP28, resulting in relative percent survivals of 92% and 39%, respectively, when compared to the control groups injected with empty vectors BmNPV and PET-30a. These results showed that VP28 protein produced in BmN cells gave much better protection than VP28 protein produced in E. coli. (c) 2006 Elsevier B.V. All rights reserved.