Simultaneous identification of Bulbus Fritillaride cirrhosae using PCR-RFLP analysis
Simultaneous identification of Bulbus Fritillaride cirrhosae using PCR-RFLP analysis
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DOI：
10.1016/j.phymed.2006.09.008
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发表时间：
2007-09-01
期刊：
影响因子：
7.9
通讯作者：
Yuan, C. S.
中科院分区：
文献类型：
--
作者：
Wang, C. Z.;Li, P.;Yuan, C. S.
贝母是我国最常用的止咳药。贝母属植物有9种，《中国药典》收载为药贝母。浙贝母（Fritillariae fritillae fritillae，BF eirrhosae）是一个由四种野生贝母组成的类群，这四种贝母与其他五种贝母相比，具有更高的效率和更低的毒性。通过外观、显微镜和化学技术进行分析具有局限性。植物资源鉴定是中药标准化的重要环节。本文测定了贝母属9种1变种的核核糖体DNA（nrDNA）内转录间隔区（ITS I）的序列。利用限制性内切酶SmaI的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，在核核糖体ITSI区发现了一个突变位点，可用于鉴别黑胫病与其它种黑胫病，是一种成功的区分亚群的方法。(c)2006年由Elsevier GmbH出版。
Bulbus Fritillariae (BF) is the most commonly used antitussive herb in China. There are nine species of Fritillaria recorded as the drug BF in the Chinese Pharmacopoeia. Bulbus Fritillariae cirrhosae (BF eirrhosae) is a group that includes four species of BF, these four species come from wild sources with higher efficiency and lower toxicity compared to the other five species of BE Due to reasons of carelessness and reduced costs, the other five species are often sold as BF cirrhosae. Analysis through appearance, microscopic and chemical techniques has limitations. Identifying botanical resources is a primary step in the standardization of herbal medicine. In the present article, the internal transcribed spacer 1 (ITS I) regions of the nuclear ribosomal DNA (nrDNA) of nine species and one variety of Fritillaria genus have been sequenced. A mutation site in the ITS I region among BF cirrhosae and other species of BF has been found and can be recognized by the restriction endonuclease SmaI Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the nuclear ribosomal ITSI region was used to differentiate BF cirrhosae from other species of BF and is a successful method in distinguishing the subgroups. (c) 2006 Published by Elsevier GmbH.