Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury.
Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury.
复制标题
肝缺血/再灌注损伤后小鼠血浆和肝脏中失调的 LncRNA 谱的比较分析。
DOI：
10.1371/journal.pone.0133462
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Chen Z;Luo Y;Yang W;Ding L;Wang J;Tu J;Geng B;Cui Q;Yang J
长链非编码RNA（longnoncodingRNA，LncRNA）被认为是多种组织和器官的主要转录本，在许多生物学过程中起着重要的调控作用。本研究利用微阵列技术测定了小鼠肝脏缺血/再灌注损伤（IRI）后血浆中LncRNA的表达谱。基因芯片检测结果显示，肝IRI小鼠血浆中有64个LncRNA表达上调，244个LncRNA表达下调。在这些失调的血浆LncRNA中，59-61%是基因间的，22-25%是反义重叠的，8-12%是有义重叠的，6-7%是双向的。通过定量PCR分析验证了10个失调的血浆LncRNA，证实了芯片分析结果的准确性。肝脏IRI后血浆和肝脏LncRNA表达谱的比较分析显示，在308个表达异常的血浆LncRNA中，有245个LncRNA存在于肝脏中，但没有变化。相反，在IRI后98种失调的肝脏LncRNA中，只有19种存在于血浆中，但保持不变。先前已经报道了IRI后LncRNA AK 139328在肝脏中上调，并且肝脏AK 139328的沉默改善了肝脏IRI。微阵列和RT-PCR分析均未能检测到小鼠血浆中AK 139328的存在。总之，本研究比较了肝脏IRI后小鼠血浆和肝脏中LncRNA表达异常的差异，提示一组血浆LncRNA表达异常有可能成为评价缺血性肝损伤的新生物标志物。
Long noncoding RNAs (LncRNAs) have been believed to be the major transcripts in various tissues and organs, and may play important roles in regulation of many biological processes. The current study determined the LncRNA profile in mouse plasma after liver ischemia/reperfusion injury (IRI) using microarray technology. Microarray assays revealed that 64 LncRNAs were upregulated, and 244 LncRNAs were downregulated in the plasma of liver IRI mouse. Among these dysregulated plasma LncRNAs, 59-61% were intergenic, 22-25% were antisense overlap, 8-12% were sense overlap and 6-7% were bidirectional. Ten dysregulated plasma LncRNAs were validated by quantitative PCR assays, confirming the accuracy of microarray analysis result. Comparison analysis between dysregulated plasma and liver LncRNA profile after liver IRI revealed that among the 308 dysregulated plasma LncRNAs, 245 LncRNAs were present in the liver, but remained unchanged. In contrast, among the 98 dysregulated liver LncRNAs after IRI, only 19 were present in the plasma, but remained unchanged. LncRNA AK139328 had been previously reported to be upregulated in the liver after IRI, and silencing of hepatic AK139328 ameliorated liver IRI. Both microarray and RT-PCR analyses failed to detect the presence of AK139328 in mouse plasma. In summary, the current study compared the difference between dysregulated LncRNA profile in mouse plasma and liver after liver IRI, and suggested that a group of dysregulated plasma LncRNAs have the potential of becoming novel biomarkers for evaluation of ischemic liver injury.