Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditions
Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditions
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DOI：
10.1182/blood-2004-05-1827
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发表时间：
2005-02-01
期刊：
影响因子：
20.3
通讯作者：
Moroi, M
中科院分区：
文献类型：
--
作者：
Tomokiyo, K;Kamikubo, Y;Moroi, M
血浆血管性血友病因子（VWF）已被确定为流动条件下血小板粘附和胶原表面血栓形成不可或缺的因子。VWF与胶原蛋白结合，通过与血小板糖蛋白Ib的相互作用将血小板拴在胶原表面，也有助于胶原表面血栓的形成。在本研究中，我们证明添加VWF/因子VIII复合物或纯化的VWF（>2瑞斯托斯汀辅助因子活性单位/mL）可使血小板减少的血液中血小板粘附到胶原表面（类似于5 × 10(4)个血小板/mL）达到正常水平。在血流开始之前，VWF被允许与胶原蛋白表面结合时，没有刺激作用。在这些流动条件下，将过量的FITC（荧光素-5-异硫氰酸酯）标记的VWF添加到血小板减少的血液中，表明VWF主要被合并到血小板聚集体中。这些结果表明，在血小板减少的情况下，补充VWF通过增强血小板聚集来刺激血小板粘附在胶原表面。这也提示了一种可能性，即对血小板减少症患者补充VWF可能对增加其止血潜能有效。(C) 2005年由美国血液病学会出版。
Plasma von Willebrand factor (VWF) has been identified as an indispensable factor for platelet adhesion and thrombus formation on a collagen surface under flow conditions. VWF binds to collagen and then tethers platelets to the collagen surface through interaction with platelet glycoprotein Ib and also contributes to the thrombus formation on the collagen surface. In the present study, we demonstrated that the addition of VWF/factor VIII complex or purified VWF (> 2 ristocetin cofactor activity units/mL) increased platelet adhesion to the collagen surface in platelet-reduced blood (similar to 5 x 10(4) platelets/muL) to the normal level. VWF had no stimulatory effect when it was allowed to bind to the collagen surface before blood flow was initiated. Addition of an excess of FITC (fluorescein-5-isothiocyanate)labeled VWF to platelet-reduced blood under these flow conditions demonstrated that the VWF was mainly incorporated into the platelet aggregates. These results indicated that the supplemented VWF stimulates the platelet adhesion onto the collagen surface by enhancing platelet aggregation in the platelet-reduced condition. This also suggests a possibility that supplementation of VWF to individuals with thrombocytopenia might be effective for increasing their hemostatic potential. (C) 2005 by The American Society of Hematology.