Transplantation of choroid plexus epithelial cells into contusion-injured spinal cord of rats.
Transplantation of choroid plexus epithelial cells into contusion-injured spinal cord of rats.
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DOI：
10.3233/rnn-150546
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发表时间：
2016-02-24
影响因子：
2.8
通讯作者：
Ide C
中科院分区：
文献类型：
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作者：
Kanekiyo K;Nakano N;Noda T;Yamada Y;Suzuki Y;Ohta M;Yokota A;Fukushima M;Ide C
目的：在脊髓损伤（SCI）大鼠模型上，观察了脉络丛上皮细胞（CPECs）移植对脊髓损伤后运动功能改善和组织修复（包括轴突延伸）的影响。研究方法：从绿色荧光蛋白（GFP）转基因大鼠脉络丛中培养CPECs，并将其直接移植到同品系大鼠的脊髓挫伤病变中。移植后每周根据BBB评分评估运动行为，直至移植后4周。移植后第2天、每周、直至第5周进行组织学和免疫组化检查。结果：细胞移植组大鼠的运动行为（BBB评分）明显改善。大量轴突生长，偶尔与CPEC相互作用，通过星形胶质细胞缺乏的地区。这些轴突具有周围神经的结构特征。移植后第2天，在病灶边缘可见GAP-43阳性轴突。与对照组相比，细胞移植组脊髓空洞形成减少。在脊髓损伤内发现CPEC，并且有时与损伤边缘的星形胶质细胞相关联，直到移植后2周。结论：移植CPECs可促进SCI大鼠的运动功能改善和组织修复，包括轴突再生。
Purpose: The effect of the transplantation of choroid plexus epithelial cells (CPECs) on locomotor improvement and tissue repair including axonal extension in spinal cord lesions was examined in rats with spinal cord injury (SCI). Methods: CPECs were cultured from the choroid plexus of green fluorescent protein (GFP)-transgenic rats, and transplanted directly into the contusion-injured spinal cord lesions of rats of the same strain. Locomotor behaviors were evaluated based on BBB scores every week after transplantation until 4 weeks after transplantation. Histological and immunohistochemical examinations were performed at 2 days, and every week until 5 weeks after transplantation. Results: Locomotor behaviors evaluated by the BBB score were significantly improved in cell-transplanted rats. Numerous axons grew, with occasional interactions with CPECs, through the astrocyte-devoid areas. These axons exhibited structural characteristics of peripheral nerves. GAP-43-positive axons were found at the border of the lesion 2 days after transplantation. Cavity formation was more reduced in cell-transplanted than control spinal cords. CPECs were found within the spinal cord lesion, and sometimes in association with astrocytes at the border of the lesion until 2 weeks after transplantation. Conclusion: The transplantation of CPECs enhanced locomotor improvement and tissue recovery, including axonal regeneration, in rats with SCI.