Cure of Helicobacter pylori infection by omeprazole-clarithromycin-based therapy in non-human primates.
Cure of Helicobacter pylori infection by omeprazole-clarithromycin-based therapy in non-human primates.
复制标题
以奥美拉唑-克拉霉素为基础的疗法治愈非人类灵长类动物中的幽门螺杆菌感染。
DOI：
10.1007/pl00009961
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发表时间：
1998
影响因子：
6.3
通讯作者：
Blaser,MJ
中科院分区：
文献类型：
--
作者：
Dubois,A;Berg,DE;Fiala,N;Heman-Ackah,LM;Perez-Perez,GI;Blaser,MJ
群体饲养的恒河猴往往在生命早期自然感染幽门螺杆菌。早期尝试治愈H.幽门螺杆菌感染采用为期 10 天的三联疗法（甲硝唑、阿莫西林和肽双酚）仅部分成功（60%），可能是因为先前存在甲硝唑耐药性。本研究的目的是确定基于克拉霉素-奥美拉唑的替代疗法治疗 H 的疗效。恒河猴（Macaca mulatta）的幽门螺杆菌感染，并检查治愈的组织学和血清学相关性。五只猴子在氯胺酮麻醉下接受内窥镜检查。胃活检的组织学和培养以及血清学测试表明它们是H.幽门螺杆菌阳性。两只动物之前没有接受过抗H.幽门螺杆菌治疗，而另外三只动物三联疗法失败并携带甲硝唑耐药性幽门螺杆菌。幽门螺杆菌。对这五只动物进行奥美拉唑、克拉霉素、阿莫西林和碱式水杨酸铋四联治疗 10 天。所有五只动物都治愈了感染，并且仍然是H。无幽门螺杆菌，基于治疗后 5 个月的定期组织学和培养，并在此期间进行随访。胃炎评分和抗 H.在此期间，每只动物的幽门螺杆菌IgG水平下降至未感染动物的特征水平。这些结果表明基于奥美拉唑-克拉霉素的治疗方案可以治愈幽门螺杆菌。恒河猴中的幽门螺杆菌感染，并解决了异常组织学和血清学反应。他们认为这种临床前动物模型可用于测试新的抗幽门螺旋杆菌。幽门疗法。
Rhesus monkeys raised in colonies tend to become naturally infected byHelicobacter pyloriearly in life. Earlier attempts to cureH. pyloriinfection with a 10-day triple therapy (metronidazole, amoxicillin, and peptobismol) were only partially (60%) successful, probably because of preexisting metronidazole resistance. This study was carried out to determine the efficacy of an alternative clarithromycin-omeprazole-based therapy for curingH. pyloriinfection in Rhesus mon-keys (Macaca mulatta), and to examine histologic and serologic correlates of curing. Five monkeys were endoscoped under ketamine anesthesia. Histology and culture of gastric biopsies and serologic tests demonstrated that they wereH. pylori-positive. Two animals had not received prior anti-H. pyloritreatment, while three other animals had failed triple therapy and carried metronidazole-resistantH. pyloristrains. Quadruple therapy with omeprazole, clarithromycin, amoxicillin, and bismuth subsalicylate was given for 10 days to these five animals. All five animals were cured of the infection, and remainedH. pylori-free, based on histology and culture at regular intervals for the 5 months post-therapy during which they were followed. Gastritis scores and anti-H. pyloriIgG levels decreased in each animal during this period to levels characteristic of uninfected animals. These results indicate that an omeprazole-clarithromycin-based regimen can cureH. pyloriinfection in Rhesus monkeys, with resolution of abnormal histology and serologic responses. They suggest that this preclinical animal model is useful for testing new anti-H. pyloritherapies.