Development of microsatellite markers for Isodon longitubus (Lamiaceae).
Development of microsatellite markers for Isodon longitubus (Lamiaceae).
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DOI：
10.3732/apps.1300028
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发表时间：
2013-10
影响因子：
3.6
通讯作者：
Maki M
中科院分区：
文献类型：
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作者：
Yamashiro T;Yamashiro A;Dohzono I;Maki M
·研究报告：为研究香茶菜与其近缘种的自然杂交，开发了微卫星标记。方法和结果：共开发了10对引物。公共汽车。从最初的筛选来看，所有10个基因座都是多态的，在Mt.中每个基因座有5到19个等位基因。石津居群中有9个位点表现多态性，每个位点有2 ~ 12个等位基因。虽然在一个群体中有一个位点是单态的，但从34个I.平均值分别为0.273 ~ 1.000和0.483 ~ 0.918。六对引物可以扩增所有三个物种在这项研究中（I。因弗莱克萨斯岛我，我。shikokianus）。结论：本研究开发的10个微卫星标记可用于分析猪的群体遗传结构。以及研究香茶菜属物种间的自然杂交。
• Premise of the study: Microsatellite markers were developed for Isodon longitubus to study the natural hybridization of the species and its congeners. • Methods and Results: A total of 10 primer sets were developed for I. longitubus. From the initial screening, all of 10 loci were polymorphic with five to 19 alleles per locus in the Mt. Ishizuchi population, whereas nine loci were polymorphic with two to 12 alleles per loci in the Toon population. Although one locus was monomorphic at one population, the observed and expected heterozygosity values estimated from 34 I. longitubus samples ranged from 0.273 to 1.000 and from 0.483 to 0.918, respectively. Six primer sets could amplify all three species examined in this study (I. inflexus, I. japonicus, and I. shikokianus). • Conclusions: The 10 microsatellite markers developed here will be useful in analyzing the population genetic structure of I. longitubus and in studying the natural hybridization between Isodon species.
影响因子：
1.4
作者：
Suzuki, Kazuo;Dohzono, Ikumi;Hiei, Kayako
通讯作者：
Hiei, Kayako
影响因子：
3.6
作者：
Yamashiro A;Yamashiro T;Tateishi Y
通讯作者：
Tateishi Y