Non-target-site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)
Non-target-site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum)
复制标题
意大利黑麦草（Lolium multiflorum）草甘膦抗性的非靶位点机制
DOI：
10.1111/wbm.12156
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发表时间：
2018
影响因子：
1.4
通讯作者：
Tominaga Tohru
中科院分区：
文献类型：
--
作者：
Kurata Kohei;Niinomi Yuki;Shimono Yoshiko;Miyashita Masahiro;Tominaga Tohru
在日本的静冈县，抗草甘膦的多花黑麦草是稻田和麦田堤坝上的一个严重问题。并对该生物型的抗性机理进行了分析。根据LD 50，抗性群体对草甘膦的抗性是敏感群体的2.8-5.0倍。耐药生物型的5-烯醇式莽草酸-3-磷酸合酶（EPSPS）基因序列在Pro 106处未显示非同义替换，并且在耐药生物型中未观察到该基因的扩增。采用液相色谱-串联质谱（LC-MS/MS）直接检测草甘膦及其代谢产物氨甲基膦酸（AMPA），研究草甘膦在土壤中的代谢和转运。在草甘膦处理的叶子或其他植物部分的生物型中均未检测到AMPA。敏感型和抗性型的吸收率分别为37.90 ± 3.63%和41.09 ± 3.36%，差异不显著。与敏感型相比，抗性生物型在草甘膦处理的叶片中保留更多的草甘膦（吸收草甘膦的91.36 ± 1.56%），而在未处理的芽（5.90 ± 1.17%）和根（2.76 ± 0.44%）中保留较少，分别为79.58 ± 3.73%，15.77 ± 3.06%和4.65 ± 0.89%。结果表明，L. L.的抗性机制既不是获得代谢系统，也不是限制草甘膦的吸收，而是限制草甘膦的转运。在静冈县的多花。
In Shizuoka Prefecture, Japan, glyphosate‐resistantLolium multiflorumis a serious problem on the levees of rice paddies and in wheat fields. The mechanism of resistance of this biotype was analyzed. Based on LD50, the resistant population was 2.8–5.0 times more resistant to glyphosate than the susceptible population. The 5‐enolpyruvyl‐shikimate‐3‐phosphate synthase (EPSPS) gene sequence of the resistant biotype did not show a non‐synonymous substitution at Pro106, and amplification of the gene was not observed in the resistant biotype. The metabolism and translocation of glyphosate were examined 4 days after application through the direct detection of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) using liquid chromatograph‐tandem mass spectrometer (LC‐MS/MS). AMPA was not detected in either biotype in glyphosate‐treated leaves or the other plant parts. The respective absorption rates of the susceptible and resistant biotypes were 37.90 ± 3.63% and 41.09 ± 3.36%, respectively, which were not significantly different. The resistant biotype retained more glyphosate in a glyphosate‐treated leaf (91.36 ± 1.56% of absorbed glyphosate) and less in the untreated parts of shoots (5.90 ± 1.17%) and roots (2.76 ± 0.44%) compared with the susceptible biotype, 79.58 ± 3.73%, 15.77 ± 3.06% and 4.65 ± 0.89%, respectively. The results indicate that the resistance mechanism is neither the acquisition of a metabolic system nor limiting the absorption of glyphosate but limited translocation of the herbicide in the resistant biotype ofL. multiflorumin Shizuoka Prefecture.