Cytotoxic T-cell activity is not detectable in Venezuelan equine encephalitis virus-infected mice
Cytotoxic T-cell activity is not detectable in Venezuelan equine encephalitis virus-infected mice
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DOI：
10.1016/s0168-1702(02)00275-7
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发表时间：
2003-02-01
期刊：
影响因子：
5
通讯作者：
Phillpotts, RJ
中科院分区：
文献类型：
--
作者：
Jones, LD;Bennett, AM;Phillpotts, RJ
Previously published research has established that the immune response to the Venezuelan equine encephalitis virus (VEEV) vaccine strain TC-83 is Th 1-mediated, with local activation of both CD4+ and CD8+ T cells. This suggests that cytotoxic lymphocytes CTL may play a role in protection against virulent VEEV. Studies involving a variety of immunisation schedules with either TC-83 or strain CAAR 508 (serogroup 5) of VEEV, and six different haplotypes of mice, failed to reveal functional CTL activity against VEEV-infected targets in secondary antigen-stimulated lymphocyte cultures from either the draining lymph nodes (LN) or spleen. Nor were VEEV-specific CTL detected after immunisation of mice (three haplotypes) with recombinant vaccinia viruses (VV) expressing either the non-structural (nsP1-4) or the structural (C-E3-E2-6K-E1) genes of TC-83. Reciprocal experiments in which mice were immunised with TC-83, and their lymphocytes tested against VV recombinant-infected targets also failed to detect CTL activity. These data suggest that VEEV infection of mice does not elicit detectable CTL activity, and that CTL are unlikely to play a role in protection against virulent VEEV. (C) 2002 Elsevier Science B.V. All rights reserved.
