The simian virus 40 core C enhancer-like element is a positive regulator in the rat alpha1B adrenergic receptor gene proximal promoter.
The simian virus 40 core C enhancer-like element is a positive regulator in the rat alpha1B adrenergic receptor gene proximal promoter.
复制标题
猿猴病毒40核心C增强子样元件是大鼠α1B肾上腺素受体基因近端启动子的正调节因子。
DOI：
10.1006/bbrc.1998.9860
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Gao,B
中科院分区：
文献类型：
--
作者：
Dao,N;Gao,B
大鼠α 1肾上腺素能受体（α1BAR）基因的转录由三个启动子（P1、P2和P3）控制，它们分别产生2.3、2.7和3.3 kb的转录物。2.3 kb mRNA的表达具有组织特异性。为了探索潜在的机制，分析了P1启动子。DNA酶I对P1启动子的足迹分析产生了三个受保护的区域：P1 f1（-49到-62）; P1 f2（-73到-90）和P1 f3（-95到-115）。P1 f3的序列分析揭示了SV 40核心C增强子样元件的存在。在凝胶迁移率变动分析中，发现P1 f3结合序列特异性蛋白，其被SV 40核心C增强子共有寡核苷酸竞争掉。P1 f3内的这种增强子样核心序列的突变显著降低了与P1 f3的特异性蛋白结合，并抑制了P1启动子活性。与P1 f3结合的蛋白质的分布受到限制。这些结果表明，P1启动子是由一个细胞类型特异性转录因子控制的，这可能是2.3 kb大鼠α1BAR mRNA表达的组织特异性原因。
Transcription of the rat α1Badrenergic receptor (α1BAR) gene is controlled by three promoters (P1, P2, and P3), which generate 2.3-, 2.7-, and 3.3-kb transcripts, respectively. The expression of the 2.3-kb mRNA species is tissue-specific. To explore the underlying mechanism, the P1 promoter was analyzed. DNase I footprinting of the P1 promoter yielded three protected regions: P1f1(−49 to −62); P1f2 (−73 to −90), and P1f3 (−95 to −115). Sequence analysis of P1f3 revealed the presence of an SV40 core C enhancer-like element. In gel mobility shift assays, P1f3 was found to bind a sequence specific protein, which was competed away by a SV40 core C enhancer consensus oligonucleotide. Mutations of this enhancer-like core sequence within P1f3 significantly reduced specific protein binding to P1f3 and inhibited P1 promoter activity. The distribution of the protein which binds to P1f3 is restricted. These findings suggest that the P1 promoter is controlled by a cell-type-specific transcription factor, which may account for the tissue-specific expression of 2.3-kb rat α1BAR mRNA species.