Effects of diazacholesterol dihydrochloride (SC-12937), an avian antifertility agent, on rat testis.
Effects of diazacholesterol dihydrochloride (SC-12937), an avian antifertility agent, on rat testis.
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二氮杂胆固醇二盐酸盐 (SC-12937)（一种禽类抗生育剂）对大鼠睾丸的影响。
DOI：
10.1002/j.1939-4640.1986.tb00930.x
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发表时间：
1986
影响因子：
--
通讯作者：
Chakraborty,J
中科院分区：
文献类型：
--
作者：
SinhaHikim,AP;Chakraborty,J
本研究旨在评估一种禽类化学消毒剂——20,25‐二氮卓胆固醇二盐酸盐（SC‐12937）对大鼠睾丸的作用。将成年雄性大鼠腹腔注射SC - 12937/kg/d 10 mg（第1组）或30 mg（第2组），或单独注射载药（第3组），持续10天，最后一次注射后24小时处死。在两种剂量下，SC - 12937治疗大鼠的睾丸中，观察到受影响的精管外观有很大范围的变化。其范围从看起来正常的精管到几乎完全萎缩的没有细胞的小管。受累小管表现为上皮内大小不等的空泡、多核巨细胞、生殖细胞脱落和小管萎缩。值得注意的是，在同一切片中存在严重受损和完全正常的精管彼此相邻。这些变化似乎与剂量有关。与服用10毫克SC - 12937的大鼠（19.6%）相比，服用30毫克SC - 12937的大鼠受影响的小管数量更多（34.6%）。支持细胞也受到这种药物的影响，并表现出细胞质空泡化，脂滴和髓样体的积累显著增加。在严重受累的小管中也观察到坏死的支持细胞。本文简要讨论了SC‐12937在大鼠体内抗生精作用的可能机制。
The present study was undertaken to evaluate the effectiveness of an avian chemosterilant, 20, 25‐diazacholesterol dihydrochloride (SC‐12937), on the rat testis. Adult male rats were injected intraperitoneally with 10 mg (Group 1) or 30 mg (Group 2) of SC‐12937/kg/d or with vehicle alone (Group 3) for 10 days, and were killed 24 hours after the last injection. A wide range of variation in the appearance of affected seminiferous tubules was observed in the testis of SC‐12937‐treated rats at both dose levels. This ranged from apparently normal‐looking seminiferous tubules to almost completely atrophied tubules with no cells. Affected tubules exhibited intraepithelial vacuoles of varying size, multinucleated giant cells, germ cell exfoliation, and tubular atrophy. The presence of severely damaged and entirely normal seminiferous tubules adjacent to one another in the same section was noteworthy. The changes appeared to be dose‐related. A greater number (34.6%) of affected tubules were observed in rats receiving 30 mg of SC‐12937 compared with the ones receiving 10 mg of this compound (19.6%). The Sertoli cells also were affected by this drug and exhibited cytoplasmic vacuolation, a marked increase in the accumulation of lipid droplets and myeloid bodies. Necrotic Sertoli cells also were observed in the severely affected tubules. The possible mechanism of antispermatogenic action of SC‐12937 in rats has been discussed briefly.