Ca2+ influences heat shock signal transduction in Pyropia haitanensis
Ca2+ influences heat shock signal transduction in Pyropia haitanensis
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DOI：
10.1016/j.aquaculture.2019.734618
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发表时间：
2020-02
期刊：
影响因子：
4.5
通讯作者：
Hongyan Zheng;Wenlei Wang;Kai Xu;Yan Xu;De-hua Ji;Chang-sheng Chen;Chaotian Xie
中科院分区：
文献类型：
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作者：
Hongyan Zheng;Wenlei Wang;Kai Xu;Yan Xu;De-hua Ji;Chang-sheng Chen;Chaotian Xie
近年来，气候变化引起的高温胁迫导致了焦螺旋藻（pyropiahaitanenisisthalli）的退化，对植物的生长产生了不利影响。haitanensisaquaculture行业。尽管heat-resistantP。海地菌株已被开发出来，但其抗逆性机制尚不清楚。在本研究中，我们研究了高温应激（32 °C）对p的Ca2+通量和钙调蛋白（CaM）含量的影响。haitanensisthalli。并分析了基因表达和蛋白丰度。高温胁迫显著增加Ca2+内流，以及cam及其编码蛋白的表达水平。然而，当维拉帕米抑制质膜Ca2+通道时，Ca2+内流、CaM表达水平、CaM丰度水平和光合活性显著降低。这些变化最终降低了p的耐热性。haitanensisthalli。此外，热休克蛋白基因（hsp22和hsp70）在高温条件下表达水平上调，而在Ca2+内流速率降低后表达水平下调。因此，由质膜的Ca2+通道产生的Ca2+信号似乎对p很重要。海地人对高温应力的反应。本文提供的数据提供了对p的机制的见解。海棠的耐热性。
Recently, high-temperature stress induced by climate change has resulted in the degradation ofPyropiahaitanensisthalli, which has adversely affected theP. haitanensisaquaculture industry. Although heat-resistantP. haitanensisstrains have been developed, the mechanism underlying the stress resistance remains unclear. In this study, we investigated the effects of high-temperature stress (32 °C) on the Ca2+flux and calmodulin (CaM) contents ofP. haitanensisthalli. Gene expression and protein abundance were also analyzed. The high-temperature stress significantly increased the Ca2+influx, as well as the expression levels ofCaMs and their encoded proteins. However, when the plasma membrane Ca2+channel was inhibited by verapamil, the Ca2+influx,CaMexpression levels, CaM abundance levels, and photosynthetic activities decreased considerably. These changes ultimately reduced the heat resistance ofP. haitanensisthalli. Additionally, the expression levels of heat shock protein genes (HSP22andHSP70) were upregulated under high-temperature conditions, whereas they were downregulated after the Ca2+influx rate decreased. Thus, the Ca2+signal generated by the plasma membrane's Ca2+channels appears to be important forP. haitanensisresponses to high-temperature stress. The data presented herein provide insights into the mechanisms responsible forP. haitanensis' heat resistance.